Protein Info for Psest_3725 in Pseudomonas stutzeri RCH2

Annotation: arginyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 579 TIGR00456: arginine--tRNA ligase" amino acids 3 to 579 (577 residues), 567.5 bits, see alignment E=1.4e-174 PF03485: Arg_tRNA_synt_N" amino acids 4 to 90 (87 residues), 85 bits, see alignment E=7e-28 PF00750: tRNA-synt_1d" amino acids 101 to 449 (349 residues), 430.5 bits, see alignment E=7.1e-133 PF05746: DALR_1" amino acids 463 to 579 (117 residues), 121.1 bits, see alignment E=4e-39

Best Hits

Swiss-Prot: 95% identical to SYR_PSEU5: Arginine--tRNA ligase (argS) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K01887, arginyl-tRNA synthetase [EC: 6.1.1.19] (inferred from 95% identity to psa:PST_0547)

Predicted SEED Role

"Arginyl-tRNA synthetase (EC 6.1.1.19)" (EC 6.1.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GQY7 at UniProt or InterPro

Protein Sequence (579 amino acids)

>Psest_3725 arginyl-tRNA synthetase (Pseudomonas stutzeri RCH2)
MKDSIRHLIQQALVRLTSEGVLPDGLTPAIQVENTKDKSHGDFASNIALMLAKPAGLKPR
DLAEKLIAALPQDAQVSKVEIAGPGFLNFFQNSDALAGRLETALSDAQLAVRKASATQRV
VVDLSSPNLAKEMHVGHLRSTIIGDAVGRVLEFLGDEVIRQNHVGDWGTQFGMLLAYLEE
KPAAAESELADLEQFYRAAKQRFDESAEFADRARELVVKLQAGDAQCLSLWTRFNDISLS
HCQKLYDRLNVKLTPADVKGESAYNSELLGIVDALREKGLLTEDNGAQCVFLDEFKNAEG
NPLPVIVQKAGGGYLYATTDLAAMRYRSQVLHADRALYFVDQRQALHFQMAFEVARRAGF
VHESMQLEHMGFGTMNGADGRPFKTRDGGTVKLIDLLDEAEQRAYALVKGKNPELDEAEL
RQIARAVGISAVKYADLSKHRTSDYRFNFELMLSFEGNTAPYLLYAYTRVASVFRKLGKG
IDESTGRIRLEAEQELALAAKLAQFGEVLNSVGEKGEPHLLCAYLYDLAGLFSSFYEHCP
ILSAEQEDQKQSRLRLAALTGKTLKQGLELLGLETLERM