Protein Info for Psest_3685 in Pseudomonas stutzeri RCH2

Annotation: OAH/OAS sulfhydrylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 2 to 420 (419 residues), 686.3 bits, E=6.5e-211
• PF01053: Cys_Met_Meta_PP
  • amino acids 4 to 420 (417 residues), 470.3 bits, E=7.3e-145
• PF01041: DegT_DnrJ_EryC1
  • amino acids 56 to 183 (128 residues), 34.6 bits, E=2.6e-12
• PF00155: Aminotran_1_2
  • amino acids 61 to 220 (160 residues), 42.8 bits, E=8.2e-15
• PF00266: Aminotran_5
  • amino acids 94 to 213 (120 residues), 30.2 bits, E=4.9e-11

Best Hits

Swiss-Prot: 55% identical to CYSD_SCHPO: Homocysteine synthase (met17) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 99% identity to psa:PST_0568)

MetaCyc: 53% identical to O-acetylhomoserine aminocarboxypropyltransferase (Rhodospirillum rubrum)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (3/6 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (2/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (2/5 steps found)
• homocysteine and cysteine interconversion (1/4 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Sequence Analysis Tools

See L0GQU7 at UniProt or InterPro

Protein Sequence (425 amino acids)

>Psest_3685 OAH/OAS sulfhydrylase (Pseudomonas stutzeri RCH2)
MKLETLAIHAGYSPDPTTRAVAVPIYQTTSYAFDDTQHGADLFDLKVPGNIYTRIMNPTT
DVLEQRVAALEGGVAALAVASGMAAITYAIQTIAEVGDNIVSVAKLYGGTYNLFAHTLPR
QGIEVRFAAHDDIAALESLIDERTKAVFCESIGNPAGNVIDLAALAEAAHRHGVPLIVDN
TVATPMLCRPFEHGADIVVHSLTKYMGGHGTSIGGIVVDSGKFPWAQHKKRFALLNTPDV
SYHGVTYTEAFGPAAFIGRCRVVPLRNMGAAISPFNSFLILQGLETLALRMERHCENALK
VAEFLQAHPQVAWVKYAGLPDHPEHELARRYMGGTPASILCFGIEGGIEAGARFIDALKL
VVRLVNIGDAKSLACHPASTTHRQLNADELARAGVSQDLIRLSIGIEHIDDILADLAQAL
SASKG