Protein Info for Psest_3663 in Pseudomonas stutzeri RCH2
Annotation: Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 75% identical to MURU_PSEPK: N-acetylmuramate alpha-1-phosphate uridylyltransferase (murU) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
KEGG orthology group: K00966, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.13] (inferred from 89% identity to psa:PST_0731)MetaCyc: 75% identical to N-acetyl-alpha-D-muramate 1-phosphate uridylyltransferase (Pseudomonas putida KT2440)
RXN-18661 [EC: 2.7.7.99]
Predicted SEED Role
"Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 2.7.7.24)
MetaCyc Pathways
- O-antigen building blocks biosynthesis (E. coli) (11/11 steps found)
- colanic acid building blocks biosynthesis (10/11 steps found)
- dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
- GDP-mannose biosynthesis (4/4 steps found)
- dTDP-β-D-fucofuranose biosynthesis (3/4 steps found)
- peptidoglycan recycling II (7/10 steps found)
- dTDP-4-O-demethyl-β-L-noviose biosynthesis (3/5 steps found)
- dTDP-6-deoxy-α-D-allose biosynthesis (2/4 steps found)
- dTDP-N-acetylthomosamine biosynthesis (2/4 steps found)
- dTDP-N-acetylviosamine biosynthesis (2/4 steps found)
- superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (9/14 steps found)
- dTDP-L-daunosamine biosynthesis (3/6 steps found)
- dTDP-sibirosamine biosynthesis (3/6 steps found)
- dTDP-α-D-mycaminose biosynthesis (2/5 steps found)
- dTDP-3-acetamido-α-D-fucose biosynthesis (2/5 steps found)
- dTDP-3-acetamido-3,6-dideoxy-α-D-glucose biosynthesis (2/5 steps found)
- dTDP-β-L-digitoxose biosynthesis (3/7 steps found)
- dTDP-β-L-olivose biosynthesis (3/7 steps found)
- dTDP-α-D-ravidosamine and dTDP-4-acetyl-α-D-ravidosamine biosynthesis (2/6 steps found)
- dTDP-D-desosamine biosynthesis (2/6 steps found)
- dTDP-β-L-4-epi-vancosamine biosynthesis (3/8 steps found)
- dTDP-β-L-megosamine biosynthesis (3/8 steps found)
- dTDP-β-L-mycarose biosynthesis (2/7 steps found)
- dTDP-α-D-forosamine biosynthesis (3/9 steps found)
- dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis (3/9 steps found)
- superpathway of enterobacterial common antigen biosynthesis (2/10 steps found)
- superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (7/19 steps found)
- superpathway of novobiocin biosynthesis (4/19 steps found)
- superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (14/33 steps found)
- superpathway of erythromycin biosynthesis (2/19 steps found)
- superpathway of megalomicin A biosynthesis (3/22 steps found)
- superpathway of dTDP-glucose-derived antibiotic building blocks biosynthesis (3/23 steps found)
KEGG Metabolic Maps
- Fructose and mannose metabolism
- Nucleotide sugars metabolism
- Polyketide sugar unit biosynthesis
- Streptomycin biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.7.7.24
Use Curated BLAST to search for 2.7.7.13 or 2.7.7.24 or 2.7.7.99
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GQ61 at UniProt or InterPro
Protein Sequence (223 amino acids)
>Psest_3663 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) (Pseudomonas stutzeri RCH2) MKAMILAAGKGERLRPLTLHTPKPLVRAAGVPLIEYHVRALAAAGFDELVINHAWLGQQI EDYLGDGQRFGVAIRYSAEGEPLETGGGIHRALGLLGDEPFLVVNGDIWTDYDFAQLRRP LAGLAHLVLVDNPPHHPKGDFSLAESAVTEPEVSGDALTYSGISVLHPALFEGCQPGAFK LAPLLRRAMADGQVSGECHAGLWVDVGTHERLAEVEQLLEARR