Protein Info for Psest_3650 in Pseudomonas stutzeri RCH2
Annotation: anthranilate synthase component I, non-proteobacterial lineages
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 84% identical to TRPE_PSEAE: Anthranilate synthase component 1 (trpE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K01657, anthranilate synthase component I [EC: 4.1.3.27] (inferred from 99% identity to psa:PST_0744)MetaCyc: 84% identical to anthranilate synthase alpha subunit (Pseudomonas aeruginosa PAO1)
Anthranilate synthase. [EC: 4.1.3.27]
Predicted SEED Role
"Anthranilate synthase, aminase component (EC 4.1.3.27)" in subsystem Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. or Tryptophan synthesis (EC 4.1.3.27)
MetaCyc Pathways
- superpathway of aromatic amino acid biosynthesis (18/18 steps found)
- superpathway of L-tryptophan biosynthesis (13/13 steps found)
- superpathway of chorismate metabolism (44/59 steps found)
- L-tryptophan biosynthesis (6/6 steps found)
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamate and L-glutamine biosynthesis (5/7 steps found)
- 4-hydroxy-2(1H)-quinolone biosynthesis (2/5 steps found)
- acridone alkaloid biosynthesis (1/4 steps found)
- superpathway of quinolone and alkylquinolone biosynthesis (2/10 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Phenylalanine, tyrosine and tryptophan biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 4.1.3.27
Use Curated BLAST to search for 4.1.3.27
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GN13 at UniProt or InterPro
Protein Sequence (494 amino acids)
>Psest_3650 anthranilate synthase component I, non-proteobacterial lineages (Pseudomonas stutzeri RCH2) MTREEFLRLAAEGHNRIPLSFETLADFDTPLSLYLKLADAPNSYLLESVQGGEKWGRYSI IGLPCRTVLRVRDHRISVTTDGIETEACEVEDPLAFVESFQQRYRVAPVEGLPRFNGGLV GYFGYDSVRYVERKLGNCPNPDPLGTPDILLMVSDAVVVFDNLAGKLHCIVLVDPSQPEA YEAGQARLQALRAKLRQSITPRLGLDFEKSNGAEPTFRSSFSREDYEQAVNRIKDYILAG DCMQVVISQRMSIPFKAAPIDLYRALRCFNPTPYMYFFNFGDFHVVGSSPEVLVRVEEGL VTVRPIAGTRPRGASEEADLALEQDLLSDAKELAEHLMLIDLGRNDVGRVAETGTVRLTE KMVIERYSNVMHIVSNVTGQLKAPLTAMDALRAILPAGTLSGAPKIRAMEIIDELEPVKR GVYGGAVGYLAWNGNMDTAIAIRTAVIKDGELHVQAGAGIVADSQPALEWEETLNKRRAM FRAVALAERDQQEN