Protein Info for PGA1_c36340 in Phaeobacter inhibens DSM 17395

Updated annotation (from data): Histidine ammonia-lyase (EC 4.3.1.3)
Rationale: Specifically important for utilizing L-Histidine. Automated validation from mutant phenotype: the predicted function (HISTIDINE-AMMONIA-LYASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: histidine ammonia-lyase HutH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01225: histidine ammonia-lyase
  • amino acids 4 to 504 (501 residues), 663.3 bits, E=1e-203
• PF00221: Lyase_aromatic
  • amino acids 10 to 469 (460 residues), 602.7 bits, E=2.3e-185

Best Hits

Swiss-Prot: 72% identical to HUTH_RUEPO: Histidine ammonia-lyase (hutH) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)

KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 86% identity to sit:TM1040_3005)

MetaCyc: 51% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]

Predicted SEED Role

"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)

MetaCyc Pathways

• L-histidine degradation II (5/5 steps found)
• L-histidine degradation VI (7/8 steps found)
• L-histidine degradation I (4/4 steps found)
• L-histidine degradation III (4/6 steps found)

KEGG Metabolic Maps

• Histidine metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.1.3

Sequence Analysis Tools

See I7F4B1 at UniProt or InterPro

Protein Sequence (507 amino acids)

>PGA1_c36340 Histidine ammonia-lyase (EC 4.3.1.3) (Phaeobacter inhibens DSM 17395)
MIEMIPGTVTLSTLEDIYRNLTPAKLASSAREPVEAAAQMVAEAAAGQEAVYGINTGFGK
LASTKIAPEDTATLQRNLILSHCCGVGEALPEDKTRLMMTLKLLSMGRGASGVRWLVIDQ
IEKMLAHGVIPVIPSQGSVGASGDLAPLAHMAAAMIGAGEATYDGTRMSSAEALKKAGLE
PIVLGPKEGLGLINGTQFSTACALAGLFDAWRMAEASMTIASLSTDAIMGSTAPLIADIH
TLRGHAGQINVAQQMREIMDGSEIRESHREGDTRVQDPYCIRCQPQVVGAALDVLRMAAK
TLEIEANAVTDNPLVLVNEGRIVSGGNFHAEYVGFAADQIALAVAEIGAIAQRRVALMVD
PTLSHDLPPFLTPDPGLNSGFMIAEVTTAALMSENKHLANPCVTDSTPTSANQEDHVSMA
AHGALRLARMNANLSVILGVEMLCAAQGVEARAPLQTSARLQDVLGMLRADIPALAEDRY
LAPDIEDASAMVRAGRVAEAAGVKVTA