Protein Info for PGA1_c36320 in Phaeobacter inhibens DSM 17395

Updated annotation (from data): Urocanate hydratase (EC 4.2.1.49)
Rationale: Specifically important for utilizing L-Histidine. Automated validation from mutant phenotype: the predicted function (UROCANATE-HYDRATASE-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: urocanate hydratase HutU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 559 TIGR01228: urocanate hydratase" amino acids 11 to 556 (546 residues), 933.1 bits, see alignment E=2.5e-285 PF17391: Urocanase_N" amino acids 15 to 137 (123 residues), 201.9 bits, see alignment E=4.1e-64 PF01175: Urocanase" amino acids 140 to 352 (213 residues), 288.4 bits, see alignment E=4.9e-90 PF17392: Urocanase_C" amino acids 355 to 549 (195 residues), 304.6 bits, see alignment E=3.7e-95

Best Hits

Swiss-Prot: 84% identical to HUTU_RHIME: Urocanate hydratase (hutU) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01712, urocanate hydratase [EC: 4.2.1.49] (inferred from 92% identity to sit:TM1040_3007)

MetaCyc: 75% identical to urocanase subunit (Pseudomonas putida)
Urocanate hydratase. [EC: 4.2.1.49]

Predicted SEED Role

"Urocanate hydratase (EC 4.2.1.49)" in subsystem Histidine Degradation (EC 4.2.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7ESE8 at UniProt or InterPro

Protein Sequence (559 amino acids)

>PGA1_c36320 Urocanate hydratase (EC 4.2.1.49) (Phaeobacter inhibens DSM 17395)
MSDPRKNTRDIFPPTGPEITAKSWMTEAPMRMMMNNLHPDVAENPHELVVYGGIGRAART
WQDFDLIVETLKNLEEDQTLMVQSGKPVGVFQTHKDAPRVLIANSNLVPHWANWDHFNEL
DKKGLMMYGQMTAGSWIYIGTQGIVQGTYETFAEAGRQHFGGSLKGKWILTGGLGGMGGA
QPLAAVFAGASCLAVECNPDSIDFRLRTKYLDEKAETLDEALQMIERWTAAGEAKSVGLL
GNAAEIFPELVKRAETGGIRPDIVTDQTSAHDPVNGYLPLGWTMGEWKERRESDKKAVEK
AARASMKVQVKAMCDFHAMGVPTVDYGNNIRQMALEEGLENAFDFPGFVPAYIRPLFCRG
IGPFRWAALSGDPEDIRKTDAKMKELFPENAGLHRWLDMAQERIAFQGLPARICWIGLGD
RHKAGLAFNEMVRTGELSAPVVIGRDHLDSGSVASPNRETESMLDGSDAVSDWPLLNALL
NTASGATWVSLHHGGGVGMGFSQHSGVVICCDGSEDADRRVGRVLWNDPATGVMRHADAG
YDIAKDCAQEHGLNLPGIL