Protein Info for PGA1_c36100 in Phaeobacter inhibens DSM 17395
Annotation: glutamate synthase [NADPH] large chain
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 65% identical to GLTB_AZOBR: Glutamate synthase [NADPH] large chain (gltB) from Azospirillum brasilense
MetaCyc: 65% identical to glutamate synthase alpha subunit (Azospirillum brasilense)
Glutamate synthase (NADPH). [EC: 1.4.1.13]
Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-citrulline biosynthesis (8/8 steps found)
- L-glutamate and L-glutamine biosynthesis (7/7 steps found)
- superpathway of L-citrulline metabolism (10/12 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13
Use Curated BLAST to search for 1.4.1.13
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See I7F1W4 at UniProt or InterPro
Protein Sequence (1510 amino acids)
>PGA1_c36100 glutamate synthase [NADPH] large chain (Phaeobacter inhibens DSM 17395) MTKYDAEWVRAEEAKRQWMAENGLYAEEEEHSSCGVGLVVSVNGKRSRKVVEAGIDALKA IWHRGAVDADGMTGDGAGIHVQIPAPFFYDQVRRTGHEPREDQLMAVGQVFLPRTDFGAQ ERCRTIVESEVLRMGYYIYGWRHVPVDVTCLGEKANATRPEIEQILISNSKGVDEETFER ELYVIRRRIEKAAAAAQVGQLYIASLSCRSIIYKGMMLAEQVAVFYPDLMDERFESAFAI YHQRYSTNTFPQWWLAQPFRMLAHNGEINTLKGNMNWMKSHEIRMASSTFGDYAEDIKPI VASGSSDSAALDSVFEVLVRAGRSAPMAKTMMVPESWSKQAVELPQAWRDMYSYCNSVME PWDGPAALAMTDGRWVCAGLDRNGLRPMRYVVTGDGLVIAGSEAGMVPIDEATVVEKGAL GPGQMLAVDMKKGKLFHDTAIKNKLASALPFGDWVKKINDLDSTLATATEKPLFSGDELR RRQIAAGYTIEELEQILAPMAEDGKETLASMGDDTPSAVLSKMYRPLSHFFRQNFSQVTN PPIDSLREYRVMSLKTRFGNLKNVLDEDSSQTEIIVLESPFVGNSQWDELVQNLNAPLAE IDCSFAPGQGSLNAALARIRAEAEEAVSSGAGHIVLTDQHSGEGRVAMPMILATSAVHSH LTRKGLRTFCSLNVRSAECIDPHYFAVLIGCGATVVNAYLAEDSLADRIDRGLLDGNLTE NVARYREAIDQGLLKIMAKMGISVISSYRGGLNFEAVGLSRAMCAEFFPGMTSRISGIGV TGIQSKLEEIHAKAWDNGQDVLPIGGFYKARKSGETHAWEATSMHMMQMACNRASFELWK QYSAKMQSNPPIHLRDLLQIKPMGEAVPIEEVESITSIRKRFVTPGMSLGALSPEAHKTL NVAMNRIGAKSDSGEGGEDPAHFVPEPNGDNPSAKIKQVASGRFGVTAEYLNQCEELEIK VAQGAKPGEGGQLPGMKVTDLIARLRHSTKGVTLISPPPHHDIYSIEDLAQLIYDLKQIN PRCKVTVKLVASSGVGTIAAGVAKAKADVILISGHNGGTGASPATSIKYAGLPWEMGLTE AHQVLAMNNLRERVTLRTDGGLRTGRDIVMAAMLGAEEYGIGTAALIAMGCIMVRQCQSN TCPVGVCTQDEALRGKFTGNADKVVNLITFYAQEVREILASIGARSLDEVIGRADLLAQV SRGSAHLDDLDLNPLLITVDGSANIVYNRDKDRNAVPDTLDKEIVRDAARFLQDGEKMQL SYAVQNTDRTVGTRTSSHIVRNFGMRNTLQSDHLTVKLQGSAGQSLGAFAAPGLKLEVSG DANDYVGKGLSGGTIVVRPPMASPLNASENTIVGNTVLYGATDGYLFAAGRAGERFAVRN SGASVVVEGCGACGCEYMTGGIAVILGSIGANFGAGMTGGMAYIYDPEGKAETMMNMESL VTCAVTVAHWEDQLKGLIERHLQETGSRKAAEILQHWENEKGNFLQICPKEMLNKLPQPL TLEATAVPAE