Protein Info for HP15_3493 in Marinobacter adhaerens HP15

Annotation: formamidopyrimidine-DNA glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 245 PF01149: Fapy_DNA_glyco" amino acids 3 to 90 (88 residues), 89.1 bits, see alignment E=5e-29 TIGR00577: DNA-formamidopyrimidine glycosylase" amino acids 3 to 244 (242 residues), 272.1 bits, see alignment E=2.6e-85 PF06831: H2TH" amino acids 105 to 195 (91 residues), 88.6 bits, see alignment E=3.3e-29 PF06827: zf-FPG_IleRS" amino acids 217 to 244 (28 residues), 39.3 bits, see alignment (E = 6.5e-14)

Best Hits

Swiss-Prot: 79% identical to FPG_MARHV: Formamidopyrimidine-DNA glycosylase (mutM) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K10563, formamidopyrimidine-DNA glycosylase [EC: 3.2.2.23 4.2.99.18] (inferred from 79% identity to maq:Maqu_3749)

MetaCyc: 53% identical to DNA-formamidopyrimidine glycosylase (Escherichia coli K-12 substr. MG1655)
DNA-formamidopyrimidine glycosylase. [EC: 3.2.2.23]; 3.2.2.23 [EC: 3.2.2.23]; 3.2.2.23 [EC: 3.2.2.23]; RXN-21279 [EC: 3.2.2.23]

Predicted SEED Role

"Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23)" in subsystem DNA Repair Base Excision (EC 3.2.2.23)

Isozymes

Compare fitness of predicted isozymes for: 4.2.99.18

Use Curated BLAST to search for 3.2.2.23 or 4.2.99.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PFZ3 at UniProt or InterPro

Protein Sequence (245 amino acids)

>HP15_3493 formamidopyrimidine-DNA glycosylase (Marinobacter adhaerens HP15)
MTVRNGSLRWPVPADLGEKLEGQVIKSVDRRAKYLFLNMDRGTVIVHLGMSGSLRIITDN
TPPLTHDHIDVALQSGVILRFNDPRRFGCWLWADSAEEHPLITHLGPEPLAPEFNGAHLF
RLSRGKNTPVKSFIMDNQVVVGAGNIYANEALFKSGIHPRRKAGRISLDRYHRLAEAIRE
TLSAAILMGGTTLRDFVNSDGKPGYFAQSLLVYGRGGQPCKECGAPLKEIRMNNRSTVYC
PRCQR