Protein Info for Psest_0356 in Pseudomonas stutzeri RCH2

Annotation: rarD protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 transmembrane" amino acids 9 to 27 (19 residues), see Phobius details amino acids 39 to 58 (20 residues), see Phobius details amino acids 73 to 92 (20 residues), see Phobius details amino acids 104 to 121 (18 residues), see Phobius details amino acids 128 to 145 (18 residues), see Phobius details amino acids 151 to 167 (17 residues), see Phobius details amino acids 176 to 197 (22 residues), see Phobius details amino acids 213 to 232 (20 residues), see Phobius details amino acids 242 to 261 (20 residues), see Phobius details amino acids 270 to 288 (19 residues), see Phobius details PF00892: EamA" amino acids 8 to 142 (135 residues), 49.7 bits, see alignment E=2.5e-17 TIGR00688: protein RarD" amino acids 8 to 260 (253 residues), 226.2 bits, see alignment E=2.4e-71

Best Hits

Swiss-Prot: 66% identical to Y485_PSEAE: Uncharacterized transporter PA0485 (PA0485) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K05786, chloramphenicol-sensitive protein RarD (inferred from 96% identity to psa:PST_3896)

Predicted SEED Role

"RarD protein, DMT superfamily transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GGU6 at UniProt or InterPro

Protein Sequence (296 amino acids)

>Psest_0356 rarD protein (Pseudomonas stutzeri RCH2)
MATANLRKGYLLGLATFSIWGMFPLYFKALEQYGALEIVTQRVIWSAVFGSLVLLVWRHP
GWWRELLAHPRRLGVLGISSVLIAANWLIYVWAVNHDRMVEASLGYYINPLVNVLLGMLV
LRERLRPLQWLAVAMATVGVSLQLLMLGEFPWISIVLALSFGSYGLLRKLAPVAALPGLV
VETWMLLPVALVWLFWFSSGTSTEPAFWTSSDALWLMAAGPVTLIPLLGFNAAARHLPYS
TLGFLQYLTPTLLLILAVQLFGEPFPVDRQLAFFCIWAGLAVYSLDAWRIMRKSAG