Protein Info for HP15_349 in Marinobacter adhaerens HP15

Annotation: phosphomethylpyrimidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K00941, hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [EC: 2.7.1.49 2.7.4.7] (inferred from 80% identity to maq:Maqu_0688)

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7)" (EC 2.7.4.7)

MetaCyc Pathways

superpathway of thiamine diphosphate biosynthesis I (9/10 steps found)
superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
hydroxymethylpyrimidine salvage (2/2 steps found)
thiamine diphosphate salvage II (4/5 steps found)
thiamine diphosphate salvage IV (yeast) (4/7 steps found)
superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)
thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (3/8 steps found)
4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (2/8 steps found)

KEGG Metabolic Maps

Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.49 or 2.7.4.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PLV7 at UniProt or InterPro

Protein Sequence (217 amino acids)

>HP15_349 phosphomethylpyrimidine kinase (Marinobacter adhaerens HP15)
MQDTRNVYGAEPVSADLIRKQLQCLADDSPIHAIKTGALGNAAVVDVLVDFIREHPGIPV
ITDPVIKAAGGGDLADDELVSAMKEKLFPVAEMITPNGVELAMLGNSEDADQAADNLLKG
GCESVLATGGHGTGIHIINTLYNHAPEPMRWEIERIGGEYHGTGCTLAAAISAGRASGLS
PRAAISQAQNYVHRAILHALEVGKGQPVPDRGILWER