Protein Info for PGA1_c03590 in Phaeobacter inhibens DSM 17395

Annotation: dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 516 PF00364: Biotin_lipoyl" amino acids 4 to 76 (73 residues), 79.5 bits, see alignment E=2.6e-26 amino acids 108 to 178 (71 residues), 72.2 bits, see alignment E=5e-24 TIGR01347: dihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex" amino acids 108 to 515 (408 residues), 562.8 bits, see alignment E=2.5e-173 PF02817: E3_binding" amino acids 221 to 251 (31 residues), 33.4 bits, see alignment (E = 8.3e-12) PF00198: 2-oxoacid_dh" amino acids 286 to 514 (229 residues), 282.2 bits, see alignment E=6e-88

Best Hits

KEGG orthology group: K00658, 2-oxoglutarate dehydrogenase E2 component (dihydrolipoamide succinyltransferase) [EC: 2.3.1.61] (inferred from 84% identity to sit:TM1040_3510)

Predicted SEED Role

"Dihydrolipoamide succinyltransferase component (E2) of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61)" in subsystem TCA Cycle (EC 2.3.1.61)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EIW9 at UniProt or InterPro

Protein Sequence (516 amino acids)

>PGA1_c03590 dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (Phaeobacter inhibens DSM 17395)
MTTEVRVPTLGESVTEATVATWFKKPGDAVAADEMLCELETDKVTVEVPAPAAGTLGEIV
AAEGETVGVDALLATIAEGGSDTAAAPATSAPAATKDAAEGDAGAATDVMVPTLGESVSE
ATVSTWFKKVGDSVAQDEMLCELETDKVSVEVPAPTAGILTEITAEEGSTVDATAKLGVI
SGGEAGAVTPTPSKGETAGGAQYTTPPAGQGGPAKDIANAPSAEKAMAEAGLSADQVQGS
GRDGRIMKDDVARAVAAAAAAPAASTSAPAAAAPVRAPVAADDAAREERVKMTRLRQTIA
KRLKDSQNTAAMLTTYNEVDMTEVMALRNEYKDLFLKKHGVKLGFMSFFTKACVHALNEV
PEVNAEIDGTDIVYKNFVHMGIAAGTPTGLVVPVIRDADAMSFADIEKAIAEKGARARDG
KLSMAEMQGGTFTISNGGVYGSLMSSPILNPPQSGILGMHKIQDRPMAINGKVEIRPMMY
LALSYDHRIVDGKGAVTFLVRVKEALEDPRRLLMDL