Protein Info for Psest_3541 in Pseudomonas stutzeri RCH2

Annotation: Outer membrane cobalamin receptor protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 620 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF07715: Plug" amino acids 43 to 144 (102 residues), 99.4 bits, see alignment E=1.7e-32 PF00593: TonB_dep_Rec" amino acids 196 to 576 (381 residues), 159.6 bits, see alignment E=2.5e-50

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 67% identity to pmk:MDS_4229)

Predicted SEED Role

"Outer membrane vitamin B12 receptor BtuB" in subsystem Coenzyme B12 biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GRN8 at UniProt or InterPro

Protein Sequence (620 amino acids)

>Psest_3541 Outer membrane cobalamin receptor protein (Pseudomonas stutzeri RCH2)
MKLSRLALAVALLPGVQVFAADAEQELPSMLITSARQAEPRAQATAANTVFTRADIERLQ
ARSVPELLRRVPGVQVSSAGGLPSLSLRGTGTAQTLVLVDGQRIASATSGFARLDYLAID
NIERVEVIRGPRSSLYGADAIGGVIQIFTRGGKTGINPEVRLAAGSEQTFQRSLSLAAGT
EQTRVHLGASLDERDGFDITRDNRGADRDNDGQRNKALHLKLDHQFDANWKTGLSLNDQR
GKNEYDDANDFEPGTPQDEFRVSSYSGYLDGQLTDMWNSRLELGRSFDRNHAVGAGSAWN
NTLLETTRHSAAWINRLQLSERQQLSVGGDWYEDQLDSATIFEEDSRDNSAFFAQHSFQG
ERFGTELGLRHDDNQQFGSHNSWNAAVSLPVGQSQRWILSYGEGFRAPTFSDLYGPPLWG
PNPDLKPETSKTYELQWRADLDATQLEAALYRTDVEDMIAWGENRMGNVSQARINGFEAS
AARELLGWQASLGVSIIDPRDRDSGHTLARRAKRTLSIDLDRTFGTLSAGAGWHVSSARY
DTIANTRELSGYGVFDLRAGWQSHPELRWEAKVNNLFDRDYALATYNRPDGTNWWDPSQN
YGYREAGRTALFAVTWTPSL