Protein Info for Psest_3527 in Pseudomonas stutzeri RCH2

Annotation: Pseudouridylate synthases, 23S RNA-specific

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 294 PF00849: PseudoU_synth_2" amino acids 90 to 236 (147 residues), 91.2 bits, see alignment E=4e-30

Best Hits

KEGG orthology group: None (inferred from 89% identity to psa:PST_0857)

Predicted SEED Role

"Similar to ribosomal large subunit pseudouridine synthase A" in subsystem Ribosome biogenesis bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPW3 at UniProt or InterPro

Protein Sequence (294 amino acids)

>Psest_3527 Pseudouridylate synthases, 23S RNA-specific (Pseudomonas stutzeri RCH2)
MSVSNSRPSTLHLPQGDWPTVLDCLCAHFPAIGRDTWLDRMARGRVLDADMQPIDAAQAY
RVGMRIHYFREVPNETPVPFEERVLHVDEHLVVADKPHFLSVMPAGEYVNETLLARLSKR
LGNADLVPIHRIDRLTAGLVLFSANRESRGAYQALFRQREINKRYEAICPALPGLEFPMR
RRLCMVPGEPFFLMREGDGEPNSETLIEVLERRGDFWRYGLSPISGKKHQLRLHMATLGA
GICNDPFYPQLLERSQRDADDYARPLKLLARGLRFRDPLSGALREFESMLQLEW