Protein Info for Psest_3523 in Pseudomonas stutzeri RCH2

Annotation: putative thymidine phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 526 TIGR02645: putative thymidine phosphorylase" amino acids 19 to 511 (493 residues), 610.1 bits, see alignment E=1.3e-187 PF02885: Glycos_trans_3N" amino acids 110 to 170 (61 residues), 34.7 bits, see alignment E=1.8e-12 PF00591: Glycos_transf_3" amino acids 179 to 320 (142 residues), 31 bits, see alignment E=3.1e-11 PF07831: PYNP_C" amino acids 445 to 504 (60 residues), 29.3 bits, see alignment E=8.1e-11

Best Hits

Swiss-Prot: 95% identical to TYPH_PSEU5: Putative thymidine phosphorylase (PST_0860) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K00758, thymidine phosphorylase [EC: 2.4.2.4] (inferred from 95% identity to psa:PST_0860)

Predicted SEED Role

"Thymidine phosphorylase (EC 2.4.2.4)" in subsystem Deoxyribose and Deoxynucleoside Catabolism (EC 2.4.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.4

Use Curated BLAST to search for 2.4.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GQF7 at UniProt or InterPro

Protein Sequence (526 amino acids)

>Psest_3523 putative thymidine phosphorylase (Pseudomonas stutzeri RCH2)
MTTISSETPINGKSPVHLALRRVAIDTYRENVAYLHRDCAACRAEGFQALAKVEIRVNGK
RILASLNLVDDPAIVGCDELGLSEDAFAQLGAEAGVPASISQAEPAASIPALHRKIAGER
LRREDFLAIMRDIAEHRYSKIELTAFVVACNQGELDREEVFFLTDAMIQVGRRLDWREHP
VVDKHCIGGIPGNRTSMLVVPIVAAHGMLCPKTSSRAITSPAGTADTMEVLANVELPFER
LCEMVREERGCLAWGGTSELSPADDVLIAVERPLSIDSPGQMVASILSKKIAAGSTHLLL
DIPIGPTAKVRSMAEAQRLRKLFEFVASRLGLTLDVVITDGRQPIGNGIGPVLEARDVMQ
VLQNDPAAPIDLRQKALRLAGRMLEFDPDVRGGDGYAIARDILDSGRALKKMHSIIAAQG
SKPFDHNHPPLARLSFDVLAERSAVVTGIDNLQLARIARLAGAPKVQGAGVDILRKLGEP
VQAGDCLYRVYADFPADLAFARQATERASGVSLGSADQVPSVFVEF