Protein Info for PGA1_c34840 in Phaeobacter inhibens DSM 17395

Annotation: putative tRNA-nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 384 PF01743: PolyA_pol" amino acids 28 to 150 (123 residues), 98.1 bits, see alignment E=5.2e-32 PF12627: PolyA_pol_RNAbd" amino acids 184 to 239 (56 residues), 38.7 bits, see alignment 7.1e-14

Best Hits

KEGG orthology group: K00970, poly(A) polymerase [EC: 2.7.7.19] (inferred from 65% identity to sit:TM1040_2891)

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.19 or 2.7.7.21 or 2.7.7.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7F3V3 at UniProt or InterPro

Protein Sequence (384 amino acids)

>PGA1_c34840 putative tRNA-nucleotidyltransferase (Phaeobacter inhibens DSM 17395)
MTRVKAKWLEDPAVQHVCEVLTKGGAEVYFVGGCVRNALLGVPVSDLDLSTNVAPQRVMA
LAEEAGIKAIPTGIDHGTVTLVYRGTPYEITTFRRDVATDGRRAVVAFADRIEEDAARRD
FTMNALYARPDGVLVDPLNGLPDLHARAVRFIGSAAQRIREDYLRSLRYFRFHAWYGNSD
AGFDPDALSAIAENLDGIGRLSRERVGSEVLKLLAAPDPAPAIAVMRQVGLLSLILPGSD
DRALGPLIHLEGDISADPLCRLAALGGDAPWEALRLSKSQAADVRLLRAGAEGTDGPATL
AYHHGVSTARNVMLLRSALFEAPIAPDVETEITKGAAAVFPLSAKDLMPDLQGPALGAAL
KTLQKDWIRSGFMLDRDALLKRLL