Protein Info for Psest_3487 in Pseudomonas stutzeri RCH2

Annotation: Nitrate/nitrite transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 495 transmembrane" amino acids 18 to 39 (22 residues), see Phobius details amino acids 54 to 71 (18 residues), see Phobius details amino acids 83 to 100 (18 residues), see Phobius details amino acids 112 to 132 (21 residues), see Phobius details amino acids 144 to 167 (24 residues), see Phobius details amino acids 173 to 195 (23 residues), see Phobius details amino acids 218 to 240 (23 residues), see Phobius details amino acids 248 to 272 (25 residues), see Phobius details amino acids 294 to 318 (25 residues), see Phobius details amino acids 325 to 344 (20 residues), see Phobius details amino acids 360 to 379 (20 residues), see Phobius details amino acids 385 to 409 (25 residues), see Phobius details amino acids 422 to 442 (21 residues), see Phobius details amino acids 448 to 467 (20 residues), see Phobius details PF07690: MFS_1" amino acids 21 to 266 (246 residues), 59.2 bits, see alignment E=1.8e-20 amino acids 300 to 472 (173 residues), 55.6 bits, see alignment E=2.1e-19

Best Hits

KEGG orthology group: K02575, MFS transporter, NNP family, nitrate/nitrite transporter (inferred from 98% identity to psa:PST_0895)

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPT0 at UniProt or InterPro

Protein Sequence (495 amino acids)

>Psest_3487 Nitrate/nitrite transporter (Pseudomonas stutzeri RCH2)
MRVSTLFEWKRPEIRALHLTWIAFYICFFVWFGMAPLASSMLKSVNWLTPEDLKLFAIAN
VALTIPARILVGMALDHWGPRRVFSVLLVVMAAPALFFAFGDSRTQLLVSRLILSSIGAS
FVVGIHMTAMWFKPKDIGFAEGFYAGWGNFGSAAAAMSLPAIAIHAFGGPEGWRWAIASS
AIIMALYGVYYWFALTDGPVGTIHRKPHKASALEVSTWADMIKLIVWTIPMVGVLGILVW
RIEGLGYLGSTGAMICYAVIALVVIFQVVQILRVNVPILKKGVPEDDKYPFNTVAALNST
YFANFGAELAVVSMLPMFFEQTWGLTPTTAGIIAASFAFVNLVARPMGGLVSDRFGNRRF
VMLAYMLGIAVGFLLMGLMNSKWPLIVAVAITVLCSVFVQGAEGATFGIIPSIKRRVTGQ
ISGMAGAYGNVGAVCYLTLFTFVTPSQFFMVIAAGAFVSFGLCLLWLKEPEGAFSDEYYV
SSVDRELEARQKIAS