Protein Info for Psest_3445 in Pseudomonas stutzeri RCH2

Annotation: Aerobic-type carbon monoxide dehydrogenase, middle subunit CoxM/CutM homologs

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00941: FAD_binding_5
  • amino acids 3 to 217 (215 residues), 155.3 bits, E=1.4e-49
• PF03450: CO_deh_flav_C
  • amino acids 226 to 322 (97 residues), 70.3 bits, E=1.4e-23

Best Hits

Swiss-Prot: 55% identical to PAOB_ECOLI: Aldehyde oxidoreductase FAD-binding subunit PaoB (paoB) from Escherichia coli (strain K12)

KEGG orthology group: K11178, xanthine dehydrogenase YagS FAD-binding subunit [EC: 1.17.1.4] (inferred from 94% identity to psa:PST_0925)

MetaCyc: 55% identical to aldehyde dehydrogenase, FAD-binding Fe-S subunit (Escherichia coli K-12 substr. MG1655)
Carboxylate reductase. [EC: 1.2.99.6]; 1.2.98.- [EC: 1.2.99.6]; 1.2.98.- [EC: 1.2.99.6]

Predicted SEED Role

"Periplasmic aromatic aldehyde oxidoreductase, FAD binding subunit YagS @ 4-hydroxybenzoyl-CoA reductase, beta subunit (EC 1.3.99.20)" (EC 1.3.99.20)

MetaCyc Pathways

• adenosine nucleotides degradation II (4/5 steps found)
• guanosine nucleotides degradation II (3/4 steps found)
• guanosine nucleotides degradation III (3/4 steps found)
• inosine 5'-phosphate degradation (3/4 steps found)
• purine nucleotides degradation II (aerobic) (8/11 steps found)
• ureide biosynthesis (5/7 steps found)
• adenosine nucleotides degradation I (5/8 steps found)
• guanosine nucleotides degradation I (2/4 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• superpathway of guanosine nucleotides degradation (plants) (3/6 steps found)
• purine nucleotides degradation I (plants) (7/12 steps found)
• superpathway of purines degradation in plants (11/18 steps found)
• caffeine degradation III (bacteria, via demethylation) (2/7 steps found)
• caffeine degradation IV (bacteria, via demethylation and oxidation) (2/10 steps found)
• theophylline degradation (1/9 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Purine metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.1.4, 1.3.99.20

Use Curated BLAST to search for 1.17.1.4 or 1.2.99.6 or 1.3.99.20

Sequence Analysis Tools

See L0GRI9 at UniProt or InterPro

Protein Sequence (328 amino acids)

>Psest_3445 Aerobic-type carbon monoxide dehydrogenase, middle subunit CoxM/CutM homologs (Pseudomonas stutzeri RCH2)
MTPFSYQRPERIDQAIALHGPQSRYIAGGTNLLDLMKENVLQPGQLIDINGLPLRDIEQT
AEGGLRIGALVSNADLAWHPQIEQRYPLLSKAILAGASPQLRNMASTGGNLLQRTRCYYF
YDTATPCNKREPGSGCSARDGRNRIHAILGASEHCVAVHPSDMCVALAALDARVHVEGPH
GQRRLELVDFHRLPGDQPQQDNVLVEGELITAIELPVEGFASHCAYLKVRDRASYAFALV
SVAAALEMDGDIIRRARIALGGVAHKPWRLEEAEELLSGKRAKPECFAAAADRLLQGAQP
LADNAFKVDLARRAIVRALTEAAGGTVR