Protein Info for HP15_3319 in Marinobacter adhaerens HP15

Annotation: deoxyuridine 5'-triphosphate nucleotidohydrolase Dut

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00576: dUTP diphosphatase
  • amino acids 13 to 151 (139 residues), 163.5 bits, E=1.3e-52
• PF00692: dUTPase
  • amino acids 19 to 151 (133 residues), 130.1 bits, E=2.1e-42

Best Hits

Swiss-Prot: 72% identical to DUT_HAHCH: Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) from Hahella chejuensis (strain KCTC 2396)

KEGG orthology group: K01520, dUTP pyrophosphatase [EC: 3.6.1.23] (inferred from 81% identity to maq:Maqu_3562)

MetaCyc: 70% identical to dUTP diphosphatase (Escherichia coli K-12 substr. MG1655)
dUTP diphosphatase. [EC: 3.6.1.23, 3.6.1.9]

Predicted SEED Role

"Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC 3.6.1.23)" in subsystem Nudix proteins (nucleoside triphosphate hydrolases) (EC 3.6.1.23)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)
• dZTP biosynthesis (3/5 steps found)
• pyrimidine deoxyribonucleotides dephosphorylation (1/3 steps found)
• tunicamycin biosynthesis (3/9 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Pantothenate and CoA biosynthesis
• Purine metabolism
• Pyrimidine metabolism
• Riboflavin metabolism
• Starch and sucrose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.23 or 3.6.1.9

Sequence Analysis Tools

See E4PRI6 at UniProt or InterPro

Protein Sequence (153 amino acids)

>HP15_3319 deoxyuridine 5'-triphosphate nucleotidohydrolase Dut (Marinobacter adhaerens HP15)
MSRKNLQVRILDDRIGSQIPFPEYATEGSAGLDLRACLDEPLTLEPGDTQLIRTGLSIHI
ADPSLAAMILPRSGLGHKHGIVLGNLVGLIDSDYQGELMVSCWNRGRTSFTVDVGERLAQ
LVLVPVVQADFEVVSEFDASARGDGGFGSTGTR