Protein Info for Psest_3438 in Pseudomonas stutzeri RCH2

Annotation: Glutamate dehydrogenase/leucine dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02812: ELFV_dehydrog_N
  • amino acids 55 to 182 (128 residues), 169.8 bits, E=2.2e-54
• PF00208: ELFV_dehydrog
  • amino acids 200 to 443 (244 residues), 304.2 bits, E=7.8e-95

Best Hits

Swiss-Prot: 65% identical to DHE4_SALTY: NADP-specific glutamate dehydrogenase (gdhA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00262, glutamate dehydrogenase (NADP+) [EC: 1.4.1.4] (inferred from 97% identity to psa:PST_0934)

MetaCyc: 65% identical to glutamate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Glutamate dehydrogenase (NADP(+)). [EC: 1.4.1.4]

Predicted SEED Role

"NADP-specific glutamate dehydrogenase (EC 1.4.1.4)" in subsystem Arginine and Ornithine Degradation or Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis or Proline Synthesis (EC 1.4.1.4)

MetaCyc Pathways

• L-glutamate biosynthesis III (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.4

Sequence Analysis Tools

See L0GQ55 at UniProt or InterPro

Protein Sequence (445 amino acids)

>Psest_3438 Glutamate dehydrogenase/leucine dehydrogenase (Pseudomonas stutzeri RCH2)
MIETVDAFLARLKQRDPHQPEFHQAVEEVVRSLWPFLEAHPHYMQAGIIERMVEPERAII
FRVPWVDDQGRVQVNRGFRIQMNSAIGPYKGGLRFHPSVNIGVLKFLAFEQVFKNSLTSL
PMGGGKGGSDFNPKGKSDNEVMRFCQSFMSELYRHIGADLDVPAGDIGVGGREIGFLFGQ
YKRLSNQFTSVLTGKGLPYGGSLIRPEATGYGCVYFAEEMLKSTHSSFEGKRVSISGSGN
VAQYAAQKVMELGGRVISLSDSGGTLHFPDGLTGEQWDYLMDLKNVRRGRLEEMGAHFGV
TYLADQRPWSLPCDIALPCATQNELDGEDARMLLKNGCVCVAEGANMPSTLEAVDLFLEA
GILYAPGKASNAGGVACSGLEMSQNAMRLHWTAGEVDTKLHSIMQSIHHACVAHGEENGR
INYVKGANIAGFVKVADAMLAQGVV