Protein Info for PGA1_c34250 in Phaeobacter inhibens DSM 17395

Annotation: AMP nucleosidase Amn

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 493 TIGR01717: AMP nucleosidase" amino acids 19 to 492 (474 residues), 667.3 bits, see alignment E=6.7e-205 PF10423: AMNp_N" amino acids 20 to 179 (160 residues), 172.4 bits, see alignment E=5.6e-55 PF01048: PNP_UDP_1" amino acids 255 to 440 (186 residues), 57.7 bits, see alignment E=1.2e-19

Best Hits

Swiss-Prot: 52% identical to AMN_ECO57: AMP nucleosidase (amn) from Escherichia coli O157:H7

KEGG orthology group: K01241, AMP nucleosidase [EC: 3.2.2.4] (inferred from 82% identity to sil:SPO3745)

MetaCyc: 52% identical to AMP nucleosidase (Escherichia coli K-12 substr. MG1655)
AMP nucleosidase. [EC: 3.2.2.4]

Predicted SEED Role

"AMP nucleosidase (EC 3.2.2.4)" in subsystem Purine conversions (EC 3.2.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DVG3 at UniProt or InterPro

Protein Sequence (493 amino acids)

>PGA1_c34250 AMP nucleosidase Amn (Phaeobacter inhibens DSM 17395)
MTTVKTPQLPAAEEFTDAAAAVARLELLYREATQFLCAEFSRILSDGPAVGSTGTTHRIR
AFYPEIRFTTSSFAQVDSRLSYGHVSAPGTYATTVTRPDLFRHYLTQQIGLLIRNHGQPV
TIAVSDTPIPVHFAVAADPSLTVPQEGAAGFTLRDVFDVPDLSTTNDDIVNGTYKASDDT
GPLALFTAQRVDYSLARLAHYTATDASHFQNHVLFTNYQFYVAEFEAYARAQLADPASGY
SSFVSTGDHEITEAEAEIPAIAKLPQMPTYHLKRKDGSGITLVNIGVGPSNAKTATDHIA
VLRPHAWLMVGHCAGLRNTQTLGDFVLGHGYLREDHVLDDDLPIWTPIPALAEIQVALED
AVAEVTELEGYDLKRIMRTGTVATIDNRNWELRDQSGPVQRLSQSRAIALDMESATIAAN
GYRFRVPYGTLLCVSDKPLHGELKLPGMASEFYHTQVARHLQIGIKAMEQLRGMPLERLH
SRKLRSFDETAFL