Protein Info for Psest_3407 in Pseudomonas stutzeri RCH2

Annotation: Type I site-specific restriction-modification system, R (restriction) subunit and related helicases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1067 PF04313: HSDR_N" amino acids 6 to 221 (216 residues), 42.8 bits, see alignment E=1.4e-14 PF04851: ResIII" amino acids 294 to 454 (161 residues), 53.1 bits, see alignment E=9.1e-18 PF18766: SWI2_SNF2" amino acids 297 to 552 (256 residues), 81.8 bits, see alignment E=1.5e-26 PF00270: DEAD" amino acids 298 to 500 (203 residues), 34.2 bits, see alignment E=5.3e-12 PF22679: T1R_D3-like" amino acids 675 to 729 (55 residues), 40.3 bits, see alignment 1.2e-13

Best Hits

KEGG orthology group: K01153, type I restriction enzyme, R subunit [EC: 3.1.21.3] (inferred from 92% identity to psa:PST_0639)

Predicted SEED Role

"Type I restriction-modification system, restriction subunit R (EC 3.1.21.3)" in subsystem Restriction-Modification System (EC 3.1.21.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.3

Use Curated BLAST to search for 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GQ26 at UniProt or InterPro

Protein Sequence (1067 amino acids)

>Psest_3407 Type I site-specific restriction-modification system, R (restriction) subunit and related helicases (Pseudomonas stutzeri RCH2)
MADSKEAQFQQDIIDALAAQGWLVGTASGFDRRTALYTEDVLGYFKEAWPERWDKFAKAN
PNNPDDVLVQKLVRELEQSGTLDVLRHGFKLPAVKVELCSFQPDHGMNPDTLKRYQCNRL
RVVPEVSYSPHAREAGSSGQSYNPRLDLVLFVNGIPTATLELKSEFKQSVENAKRQYRQD
RPVKDPLTRKPEPLLTFKRGALVHFAVGQNEVAMTTKLAGKDTFFLPFNLGSAEGGAGNP
MAEDDSQYATGYLWQRLLQPDAWLKVLGRFLHLEKKTSDGFDGTPVTKETMIFPRYHQWE
VVNTLINTTRAEGPGKRYLIQHSAGSGKSNSIAWTAHQLASLYDEAGQRLFNSVIVITDR
TVLDKQLQDTIYQFEHAQGVVKQINRETSSQSKSEQLAEALAEQTRIIIVTIQTFPALFD
ALDKYPKLASGRYAVIADEAHSSQTGSSASKLKQILGSDALEGEEISAEELLDAAVQARQ
PNERISYYAFTATPKAKTLELFGRPADPTLPPSASNKPEAFHLYSMRQAIEEGFILDVLR
NYTTYSTAWKIAHPDGEDDEVDSKKARIKLARWVRLHPYNISQKVEVIVEHFRANIRGLL
NGQAKAMVVTSSRQEAVRYQLALKAYVQQMGYNDVHPLVAFSGSVLPDEVIPEEVTESSS
LLNAGLNGRDLAHAFDTQDFNVMIAANKYQTGFDQPKLCAMYVDKKLQGVDCVQTLSRLN
RTFGDSKETFILDFFNEPQDILDAFLPYYTKAELTDVTDPQIIYDLQKKLDAEGIYHWQE
VEAFAMAFFDPKAGGSKLNYYSKPAKERFAKRYAFSVESRQQALDFKRTAETNGDSAGLK
KAEHALKEAGEQVDQLDLFRKNLQSFVRLYEFLSQIVPYEDRELEQLCVYAKHLHPLLRV
DRLLQDDVDVGELQLSHYRLSKRAEHQLRLSEEDGDYSLKPGSDVGSGKPHDPEKKRLSE
IIEALNDIFGAEVNDEDQLQFLTGIAQRISRQEDVMAQVNNHSVEQVMHGLFPKRVLDTV
LDAMTDHEKLSLEVLDNETKSRAFAMVILKMLTAAGGLGKSNSGAAV