Protein Info for Psest_3384 in Pseudomonas stutzeri RCH2

Annotation: glutamate 5-kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01027: glutamate 5-kinase
  • amino acids 10 to 370 (361 residues), 435 bits, E=1.2e-134
• PF00696: AA_kinase
  • amino acids 10 to 238 (229 residues), 158.2 bits, E=3e-50
• PF01472: PUA
  • amino acids 281 to 354 (74 residues), 74.1 bits, E=6.8e-25

Best Hits

Swiss-Prot: 98% identical to PROB_PSEU5: Glutamate 5-kinase (proB) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K00931, glutamate 5-kinase [EC: 2.7.2.11] (inferred from 98% identity to psa:PST_0959)

MetaCyc: 43% identical to glutamate 5-kinase (Escherichia coli K-12 substr. MG1655)
Glutamate 5-kinase. [EC: 2.7.2.11]

Predicted SEED Role

"Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA" in subsystem Proline Synthesis (EC 2.7.2.11)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-ornithine biosynthesis II (3/3 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-N^δ-acetylornithine biosynthesis (5/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.2.11

Sequence Analysis Tools

See L0GRD1 at UniProt or InterPro

Protein Sequence (372 amino acids)

>Psest_3384 glutamate 5-kinase (Pseudomonas stutzeri RCH2)
MRDKVSGARRWVVKIGSALLTADGRGLDQAAMAVWVDQMVALREAGVELVLVSSGAVAAG
MSRLGWSSRPKAVHELQAAAAVGQMGLIRAWESSFARCDQQTAQILVTHDDLSDRKRYLN
ARSTLRTLIDLGVVPVINENDTVVTDEIRFGDNDTLGALVANLVEADLLVILTDRDGMFD
ADPRFNPQANLISEARADDPALDAVAGGTGGALGRGGMQTKLRAARLAARSGAHTVIVGG
RIEQVLARLKAGERLGTLLAPECSRHAARKQWLAGHLQTRGTLTLDDGAVHALRQGNRSL
LPVGVRAVQGAFRRGEMVVCVGPQGAEVARGLVNYSAAEAQRILGRPSDEIEKLLGYVDE
PELIHRDNMILV