Protein Info for Psest_3379 in Pseudomonas stutzeri RCH2

Annotation: isoleucyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 943 TIGR00392: isoleucine--tRNA ligase" amino acids 15 to 849 (835 residues), 913.9 bits, see alignment E=5.5e-279 PF00133: tRNA-synt_1" amino acids 28 to 646 (619 residues), 457.6 bits, see alignment E=1.1e-140 PF09334: tRNA-synt_1g" amino acids 58 to 198 (141 residues), 40.8 bits, see alignment E=3.8e-14 amino acids 581 to 637 (57 residues), 20.8 bits, see alignment 4.5e-08 PF08264: Anticodon_1" amino acids 691 to 847 (157 residues), 103 bits, see alignment E=4.4e-33 PF06827: zf-FPG_IleRS" amino acids 905 to 931 (27 residues), 36.6 bits, see alignment (E = 9.3e-13)

Best Hits

Swiss-Prot: 85% identical to SYI_AZOVD: Isoleucine--tRNA ligase (ileS) from Azotobacter vinelandii (strain DJ / ATCC BAA-1303)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 85% identity to avn:Avin_11780)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GRC5 at UniProt or InterPro

Protein Sequence (943 amino acids)

>Psest_3379 isoleucyl-tRNA synthetase (Pseudomonas stutzeri RCH2)
MTDYKATLNLPSTAFPMKAGLPQREPEILQRWNSIDLYRKLRQIGEGRPKFVLHDGPPYA
NGNIHIGHAVNKVIKDMIVRSRTLSGFDAPYVPGWDCHGLPIEHKVETTFGKNQPADLTR
ERCRAYAAEQIEGQKADFIRLGVLGDWDNPYKTMDFANEAGEIRALAKLVEGGFVFKGLK
PVNWCFDCGSALAEAEVEYQDKKSDAIDVAFEVEDADKLAAAFGLPNLAKPASIVIWTTT
PWTIPANQALNVHPEFVYALVDTGERLLLLAEELVESCLQRYGLAGEIIARCEGKALELI
RFRHPFYERFAPVYLADYVETGAGTGIVHSAPAYGEDDFRSCKHYGMENDDILGPVQSNG
VYVSDLPFFGGQFIWKANPAIVEKLAEVGALLKHESIQHSYMHCWRHKTPLIYRATAQWF
VGMDKVAHDGSSLRRRALDAIEQTEFVPAWGQARLHGMIAGRPDWCISRQRTWGVPIPFF
LHKESGELHPRTVELMEQVAQRVEQGGIEAWSKLDAAELLGEEAAQYEKITDTLDVWFDS
GTTHWHVMRGSHPMGHESGPRADLYLEGSDQHRGWFHSSLLTGAAIDGHAPYKGLLTHGF
TVDENGRKMSKSLGNVIAPQEITDSMGADILRLWVSATDYSGEMAVSKQILQRSADAYRR
IRNTARFLLSNLDGFDPAQHMLPNDQLIALDRWAIDRALLLQQEIEEAYTTYRFWNVYQK
VHNFCVQELGGFYLDIIKDRQYTTGADSLPRRSCQTALYHIAEALVRWIAPILAFTAEEI
WQYLPGERNESVMLNTWYTGLTELPQDAELDRAFWDKVMAVKTAVNKELENQRAAKTIGG
NLQAEVTLYAEDALAAELAKLGNELRFVLITSAVDIAPLTQAPAEAVESELVGLKLVVKK
TGHSKCGRCWHHLPDVGSHAEHPEICGRCVENIEGAGEVRHYA