Protein Info for PGA1_c03400 in Phaeobacter inhibens DSM 17395

Updated annotation (from data): acetyl-CoA:acetyl-CoA C-acetyltransferase / acetyl-CoA:propanoyl-CoA 2-C-acetyltransferase (EC 2.3.1.9; EC 2.3.1.16)
Rationale: Specifically important for utilizing Potassium acetate amd threonine. EC 2.3.1.9 (acetoacetyl-CoA) was linked to acetate via a SEED subsystem. The phenotype on threonine suggests that propanyl-CoA is also a substrate, hence the broader EC # 2.3.1.16.
Original annotation: acetyl-CoA acetyltransferase PhaA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 80% identical to THIL_PARDE: Acetyl-CoA acetyltransferase (phaA) from Paracoccus denitrificans

KEGG orthology group: K00626, acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (inferred from 93% identity to sit:TM1040_3735)

MetaCyc: 87% identical to acetyl-CoA acetyltransferase (Cereibacter sphaeroides)
Acetyl-CoA C-acyltransferase. [EC: 2.3.1.16, 2.3.1.9]

Predicted SEED Role

"Acetyl-CoA acetyltransferase (EC 2.3.1.9) of ethylmalonyl-CoA pathway" (EC 2.3.1.9)

MetaCyc Pathways

oleate β-oxidation (30/35 steps found)
fatty acid salvage (6/6 steps found)
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (5/5 steps found)
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
benzoyl-CoA biosynthesis (3/3 steps found)
ketolysis (3/3 steps found)
(2S)-ethylmalonyl-CoA biosynthesis (3/4 steps found)
fatty acid β-oxidation I (generic) (5/7 steps found)
2-methyl-branched fatty acid β-oxidation (10/14 steps found)
superpathway of glyoxylate cycle and fatty acid degradation (10/14 steps found)
polyhydroxybutanoate biosynthesis (2/3 steps found)
L-isoleucine degradation I (4/6 steps found)
pyruvate fermentation to butanol II (engineered) (4/6 steps found)
1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
acetoacetate degradation (to acetyl CoA) (1/2 steps found)
valproate β-oxidation (6/9 steps found)
fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
glutaryl-CoA degradation (3/5 steps found)
ketogenesis (3/5 steps found)
ethylmalonyl-CoA pathway (7/11 steps found)
pyruvate fermentation to hexanol (engineered) (7/11 steps found)
acetyl-CoA fermentation to butanoate (4/7 steps found)
5,6-dehydrokavain biosynthesis (engineered) (6/10 steps found)
propanoate fermentation to 2-methylbutanoate (3/6 steps found)
superpathway of Clostridium acetobutylicum acidogenic fermentation (5/9 steps found)
4-hydroxybenzoate biosynthesis III (plants) (2/5 steps found)
3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
pyruvate fermentation to butanoate (3/7 steps found)
superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) (5/10 steps found)
glycerol degradation to butanol (9/16 steps found)
ethylbenzene degradation (anaerobic) (1/5 steps found)
fatty acid β-oxidation VII (yeast peroxisome) (1/5 steps found)
isopropanol biosynthesis (engineered) (1/5 steps found)
pyruvate fermentation to acetone (1/5 steps found)
2-deoxy-D-ribose degradation II (3/8 steps found)
pyruvate fermentation to butanol I (3/8 steps found)
(8E,10E)-dodeca-8,10-dienol biosynthesis (5/11 steps found)
mevalonate pathway I (eukaryotes and bacteria) (2/7 steps found)
mevalonate pathway II (haloarchaea) (2/7 steps found)
3-phenylpropanoate degradation (4/10 steps found)
9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
L-glutamate degradation V (via hydroxyglutarate) (4/10 steps found)
10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) (1/6 steps found)
4-ethylphenol degradation (anaerobic) (1/6 steps found)
benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
2-methylpropene degradation (2/8 steps found)
isoprene biosynthesis II (engineered) (2/8 steps found)
mevalonate pathway III (Thermoplasma) (2/8 steps found)
mevalonate pathway IV (archaea) (2/8 steps found)
L-lysine fermentation to acetate and butanoate (3/10 steps found)
superpathway of Clostridium acetobutylicum solventogenic fermentation (5/13 steps found)
4-oxopentanoate degradation (2/9 steps found)
L-glutamate degradation VII (to butanoate) (4/12 steps found)
methyl tert-butyl ether degradation (2/10 steps found)
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (7/17 steps found)
L-tryptophan degradation III (eukaryotic) (5/15 steps found)
10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
crotonate fermentation (to acetate and cyclohexane carboxylate) (4/16 steps found)
docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
benzoate fermentation (to acetate and cyclohexane carboxylate) (4/17 steps found)
toluene degradation VI (anaerobic) (4/18 steps found)
jasmonic acid biosynthesis (4/19 steps found)
cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
sitosterol degradation to androstenedione (1/18 steps found)
androstenedione degradation I (aerobic) (6/25 steps found)
platensimycin biosynthesis (6/26 steps found)
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (3/22 steps found)
superpathway of testosterone and androsterone degradation (6/28 steps found)
superpathway of ergosterol biosynthesis I (4/26 steps found)
androstenedione degradation II (anaerobic) (4/27 steps found)
Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)
superpathway of L-lysine degradation (10/43 steps found)
superpathway of cholesterol degradation I (cholesterol oxidase) (8/42 steps found)
superpathway of cholesterol biosynthesis (4/38 steps found)
superpathway of cholesterol degradation II (cholesterol dehydrogenase) (9/47 steps found)
superpathway of cholesterol degradation III (oxidase) (5/49 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.16, 2.3.1.9

Use Curated BLAST to search for 2.3.1.16 or 2.3.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DX90 at UniProt or InterPro

Protein Sequence (391 amino acids)

>PGA1_c03400 acetyl-CoA:acetyl-CoA C-acetyltransferase / acetyl-CoA:propanoyl-CoA 2-C-acetyltransferase (EC 2.3.1.9; EC 2.3.1.16) (Phaeobacter inhibens DSM 17395)
MTNVVIASAARTAVGSFGGAFAKTPAHDLGAAVLQAVVERAGIDKSEVSETILGQVLTAA
QGQNPARQAHINAGLPQESAAWSLNQVCGSGLRAVALAAQHIQLGDAAIVCAGGQENMTL
SPHAANLRAGHKMGDMSYIDTMIRDGLWDAFNGYHMGQTAENVAEKWQISREMQDEFAVA
SQNKAEAAQKAGKFADEIAAFTVKTRKGDIIVDQDEYIRHGATIEAMQKLRPAFAKDGSV
TAANASGLNDGAAATLLMSADDAEKRGIEPLARIASYATAGLDPSIMGVGPIYASRKALE
KAGWSVDDLDLVEANEAFAAQACAVNKDMGWDPAIVNVNGGAIAIGHPIGASGCRVLNTL
LFEMKRRDAKKGLATLCIGGGMGVAMCVERP