Protein Info for PGA1_c33400 in Phaeobacter inhibens DSM 17395

Annotation: leucyl-tRNA synthetase LeuS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 868 TIGR00396: leucine--tRNA ligase" amino acids 7 to 867 (861 residues), 933 bits, see alignment E=8.2e-285 PF00133: tRNA-synt_1" amino acids 13 to 246 (234 residues), 93.9 bits, see alignment E=1.9e-30 amino acids 421 to 581 (161 residues), 23.4 bits, see alignment E=4.7e-09 amino acids 639 to 678 (40 residues), 30.1 bits, see alignment 4.6e-11 PF09334: tRNA-synt_1g" amino acids 36 to 172 (137 residues), 56.9 bits, see alignment E=3.3e-19 amino acids 637 to 684 (48 residues), 23.8 bits, see alignment 3.6e-09 PF13603: tRNA-synt_1_2" amino acids 220 to 370 (151 residues), 191.5 bits, see alignment E=2.1e-60 PF08264: Anticodon_1" amino acids 712 to 832 (121 residues), 68.2 bits, see alignment E=1.5e-22

Best Hits

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 73% identity to rcp:RCAP_rcc00257)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7E566 at UniProt or InterPro

Protein Sequence (868 amino acids)

>PGA1_c33400 leucyl-tRNA synthetase LeuS (Phaeobacter inhibens DSM 17395)
MPRYVAPEIEARWQDAWEKAGIFKAKRSADKPKYYVLEMFPYPSGKLHMGHVRNYTMGDV
IARYKLSTGHSVLHPMGFDAFGMPAENAAMASGGHPKDWTYANIGTMVEQMKPLGLSLDW
SRMFATCDESYYGQQQALFLDFLEKGLVYRKNAVVNWDPVDMTVLANEQVEGGRGWRSGA
LVERRELTQWFFKISDYSDELLTALDTLENWPAKVRLMQENWIGKSRGLQFSFVRTDGGD
PIEVYTTRPDTLNGASFVGISPDHPIAKALEAESAEVAAFVAECRKGGTTEEAIETAEKL
GYDTGIRVKHPLNPDWELPVWIANFILMDYGTGAIFACPAHDQRDYEFATKYDLPIIPVF
ENPEDDSPLTEAYVPTKAEKVRYIRGFAGDDDQTGEDAVNAAVDKAEAEGWGEGVTKFRL
RDWGLSRQRYWGCPIPVVHCPDCGVVPEKKENLPIALPYDEDGKAIDFSVPGNPLDRHPS
WRNCACPACGKPAQRETDTMDTFVDSSWYFARFTAPDADTPTRMEDAEYWMNVDQYIGGV
EHAILHLLYSRFFARAMQICGHLPEKAIEPFDALFTQGMVTHAIYATWEDTGRFTKLEDG
HEEPIYGYKYHYPEEVEFRDGKTFLKDGGAEVEVIPSAKMSKSKNNVVDPLHIISSYGAD
TARWFVLSDSPPERDVEWTASGAEAAYKHLNRVWNLCDRIGEMNSGASGNGDEDLLREMH
KAIRDVTLSIETFGFNAAIAKLYSFTNTLAKSKAGTAAQKQAIMTLAQLMSPMTPHLAED
IWNHQGGEGLCATAPWPVADESMLVEDSVTLPIQINGKRRGEITVAKDLAKDEVEKIALA
HEAVQKALNGSAPKKVIVVPGRIVNVVA