Protein Info for Psest_3345 in Pseudomonas stutzeri RCH2

Annotation: Sulfite reductase, alpha subunit (flavoprotein)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00258: Flavodoxin_1
  • amino acids 59 to 181 (123 residues), 97.1 bits, E=1.1e-31
• PF00175: NAD_binding_1
  • amino acids 377 to 482 (106 residues), 47 bits, E=3.8e-16

Best Hits

KEGG orthology group: K00380, sulfite reductase (NADPH) flavoprotein alpha-component [EC: 1.8.1.2] (inferred from 86% identity to psa:PST_0997)

Predicted SEED Role

"probable oxidoreductase"

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.2

Use Curated BLAST to search for 1.8.1.2

Sequence Analysis Tools

See L0GR22 at UniProt or InterPro

Protein Sequence (508 amino acids)

>Psest_3345 Sulfite reductase, alpha subunit (flavoprotein) (Pseudomonas stutzeri RCH2)
LSPLLGCLAVAALLLWWQPPRAISAAVVVLVYLAMSLYCWRGQLRRKAASTDATSALVVT
YASQGGQARLYAERSAEQLREAGMNVQLLPLNALEPSRLPARRILFVVSTYGEGEAPDNG
ARFEQRLNAANLQLQDLEYSVLAFGDRQYRDFCAFGRRLDERLRQLGGVPLFDRLEADRG
DPGVLRHWQHQLAHLSGNSNFSDWLPAPYQPWRLTGRRCLNAGSSGAPVHLLTLAPPSEK
TSWRAGDIVEIGPRHALARIEALLHDVGLDPSALVDGQSLAAQLSRRHLPATGDLHGVDA
EALLALPPLPHREYSIASIPADGAVQLVVREAFHSDGTPGLGSGWLCRYAAIGEEIDLRI
RSNPSFHGRDAATPLILIGNGTGIAGLRAHLRERAAQPGSRNWLLFGERNAAHDFLFHEE
LAQWRESGHLQRLDVAFSRDQPQKRYVQDALRDAADELRCWVDQGAAIYVCGSLEGMGQE
VQQILLGLLGEAQLEQLSEQGRYRRDLY