Protein Info for PGA1_c33130 in Phaeobacter inhibens DSM 17395

Annotation: dimethylglycine dehydrogenase DmgdH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 815 PF01266: DAO" amino acids 9 to 378 (370 residues), 220.4 bits, see alignment E=1.3e-68 PF16350: FAO_M" amino acids 381 to 435 (55 residues), 60 bits, see alignment 5.1e-20 PF01571: GCV_T" amino acids 438 to 701 (264 residues), 196.1 bits, see alignment E=1.7e-61 PF08669: GCV_T_C" amino acids 727 to 807 (81 residues), 60.7 bits, see alignment E=2.6e-20

Best Hits

KEGG orthology group: K00315, dimethylglycine dehydrogenase [EC: 1.5.99.2] (inferred from 83% identity to sil:SPO3400)

Predicted SEED Role

"Sarcosine dehydrogenase (EC 1.5.99.1)" (EC 1.5.99.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.99.1, 1.5.99.2

Use Curated BLAST to search for 1.5.99.1 or 1.5.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7ERI3 at UniProt or InterPro

Protein Sequence (815 amino acids)

>PGA1_c33130 dimethylglycine dehydrogenase DmgdH (Phaeobacter inhibens DSM 17395)
MSDFPTKARVVIIGGGVVGCSALYHLAKKGWADCVLLEKNELTAGSTWHAAGNVPTFSTS
WAIMNMQRYSTELYAGLGEEVDYPMNYHQSGSIRLAHTKERMQEFERACSMGRYQGIEME
MWTPDQAKEHYPFLETHDLEGVLYDPTDGDIDPAQVTQALAKGARDMGARIIRFCPATGV
TQKEDKTWIVQTDKGDIECDYVVNAAGYYAQRVGEWFKDYGGRTVPMMVMSHQYLLTEQI
PEIEAWSKEHGKKLPLIRDVDVSYYLRQEKNGYNLGPYEPNCRGHWMTEDDQMPDDFSFQ
LWSDDLDRIEDIVTDAMERVPLMATSGVSSVINGPIPYAPDGLPLIGPMPGVDNAFEACV
FTFGIAQGGGAGKVLAEWIVDGQTEWDMWAVDPRRYTDYTDQDYCDQKGMEVYGNEYAMH
FPHHEWPAARDKKMSQVHATIKALGGVMGAYNGWERANWFAKPGDDTSEEATHTWGRSGP
WQQRIKEECEAVRDGVGVLDLPGFSRFNLSGEGAAEFLRGLVTGGLPKVGRMNLVYVSDD
RGRILTEMSCIRHGEDHFTMITAGSAQWHDFEILKKALPAGLRLTDHTTEFATMIVTGPQ
SRDLFAGISDADLSLGWLTHQEATVAGKPAFLARVSYAGELGWEVHCANEHQPAIYDALL
AGGAKPFGMYALNSLRIEKGYRTWKGDLSTDYSLLEGGLERFVKLDKPQDFPGKAAIQSE
KQQGVKKSFVTLIVEAGDADAPYMSCIWKDGEIVGETTSGDWGYRVNASIALGMVRSDAA
VPGTELEVEIYGEKFRAVVQEDKPLWDPGNDRLRA