Protein Info for Psest_3308 in Pseudomonas stutzeri RCH2

Annotation: KpsF/GutQ family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 324 PF01380: SIS" amino acids 40 to 172 (133 residues), 107 bits, see alignment E=6.3e-35 TIGR00393: sugar isomerase, KpsF/GutQ family" amino acids 45 to 313 (269 residues), 370 bits, see alignment E=4e-115 PF00571: CBS" amino acids 201 to 258 (58 residues), 33.6 bits, see alignment E=4.2e-12 amino acids 269 to 321 (53 residues), 30.9 bits, see alignment 2.7e-11

Best Hits

Swiss-Prot: 80% identical to KDSD_PSEAE: Arabinose 5-phosphate isomerase KdsD (kdsD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K06041, arabinose-5-phosphate isomerase [EC: 5.3.1.13] (inferred from 96% identity to psa:PST_1036)

MetaCyc: 60% identical to D-arabinose 5-phosphate isomerase KdsD (Escherichia coli K-12 substr. MG1655)
Arabinose-5-phosphate isomerase. [EC: 5.3.1.13]

Predicted SEED Role

"Arabinose 5-phosphate isomerase (EC 5.3.1.13)" in subsystem KDO2-Lipid A biosynthesis (EC 5.3.1.13)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.3.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GR53 at UniProt or InterPro

Protein Sequence (324 amino acids)

>Psest_3308 KpsF/GutQ family protein (Pseudomonas stutzeri RCH2)
MSQSSQLIETAQRTIRLEIEAVEQLNARIDASFVSACELILACKGRVVVVGMGKSGHIGR
KIAATLASTGTAAFFVHPAEASHGDMGMITQDDVVLALSNSGTTSEIVTLLPLIKRLGIT
LISMTGNPNSVLAKAAAVNLDASVSIEACPLNLAPTSSTTASLVLGDALAIALLEARGFT
AEDFAFSHPGGALGRRLLLKVEHVMHTGERLPRVPRGTSLRDALLEMTQKGLGMTVIVET
DGRLAGIFTDGDLRRALDKGVDVRQTSIDEVMTVHGKTANAEMLAAEALKIMEDHKISSL
VVIDDSELPVGALNMHDLLRAGVM