Protein Info for Psest_3288 in Pseudomonas stutzeri RCH2

Annotation: type IV-A pilus assembly ATPase PilB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 567 TIGR02538: type IV-A pilus assembly ATPase PilB" amino acids 10 to 567 (558 residues), 840.6 bits, see alignment E=3e-257 PF05157: MshEN" amino acids 62 to 147 (86 residues), 64 bits, see alignment E=1e-21 PF00437: T2SSE" amino acids 179 to 563 (385 residues), 514.4 bits, see alignment E=1.4e-158

Best Hits

Swiss-Prot: 79% identical to PILB_PSEAE: Type 4 fimbrial assembly protein PilB (pilB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02652, type IV pilus assembly protein PilB (inferred from 93% identity to psa:PST_1055)

Predicted SEED Role

"Type IV fimbrial assembly, ATPase PilB" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GR38 at UniProt or InterPro

Protein Sequence (567 amino acids)

>Psest_3288 type IV-A pilus assembly ATPase PilB (Pseudomonas stutzeri RCH2)
MSENISLSGLARQVVGAGVLDERTAQQAQAQALRSKTPFVTYAVQNRLVNGRVIAELASE
QFGVALLDLGALDKELLPKDLISEKLAKQHRVLPLSKRGTKLFIAISDPANHQSIADIQF
STGLSIEPILVEDDKLGEAIDKLYDNGSTGLEDLGDVDLDGVDVESVSDDGKGDEAGETA
DDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAFRVRLRTDGILHEVARPPIQLAPRISAR
LKVMAGLDISERRRPQDGRIKMKLSKNKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGID
ALGYEEDQKELYMNALKQPQGMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEI
NLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRDLETAEIAIKAAQTGHMVMST
LHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEVELPREALLGEGFP
ADKIGAFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGF
NDLRTSALVKAMQGVTSLEEVNRVTKD