Protein Info for Psest_0323 in Pseudomonas stutzeri RCH2

Annotation: S-adenosylmethionine synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00438: S-AdoMet_synt_N
  • amino acids 6 to 101 (96 residues), 139.5 bits, E=7.2e-45
• TIGR01034: methionine adenosyltransferase
  • amino acids 6 to 393 (388 residues), 596.1 bits, E=1.3e-183
• PF02772: S-AdoMet_synt_M
  • amino acids 115 to 232 (118 residues), 154 bits, E=2.8e-49
• PF02773: S-AdoMet_synt_C
  • amino acids 234 to 366 (133 residues), 221.8 bits, E=4.4e-70

Best Hits

Swiss-Prot: 98% identical to METK_PSEU5: S-adenosylmethionine synthase (metK) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K00789, S-adenosylmethionine synthetase [EC: 2.5.1.6] (inferred from 98% identity to psa:PST_3926)

MetaCyc: 73% identical to methionine adenosyltransferase (Escherichia coli K-12 substr. MG1655)
Methionine adenosyltransferase. [EC: 2.5.1.6]

Predicted SEED Role

"S-adenosylmethionine synthetase (EC 2.5.1.6)" in subsystem Methionine Biosynthesis or Methionine Degradation or Quorum Sensing: Autoinducer-2 Synthesis (EC 2.5.1.6)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• S-adenosyl-L-methionine salvage II (3/3 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• S-adenosyl-L-methionine biosynthesis (1/1 steps found)
• L-methionine degradation I (to L-homocysteine) (2/3 steps found)
• S-adenosyl-L-methionine salvage I (2/4 steps found)
• ethene biosynthesis I (plants) (1/3 steps found)
• superpathway of L-cysteine biosynthesis (mammalian) (2/5 steps found)
• 2'-deoxymugineic acid phytosiderophore biosynthesis (1/4 steps found)
• L-methionine salvage cycle III (6/11 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (2/7 steps found)
• L-methionine salvage cycle I (bacteria and plants) (5/12 steps found)
• L-methionine salvage cycle II (plants) (4/11 steps found)
• superpathway of L-methionine salvage and degradation (5/16 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism
• Selenoamino acid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.6

Sequence Analysis Tools

See L0GHZ7 at UniProt or InterPro

Protein Sequence (396 amino acids)

>Psest_0323 S-adenosylmethionine synthetase (Pseudomonas stutzeri RCH2)
MSEYSIFTSESVSEGHPDKIADQISDAVLDAIIAEDKHARVACETLVKTGVAIVAGEVTT
SAWVDLEQLVRDVIIDIGYNSSEVGFDGATCGIINIIGKQSVDIAQGVDRSKPEDQGAGD
QGLMFGYASNETDVLMPAPIRFSHALVERQAEARKNGLLPWLRPDAKSQVTCRYENGKVA
GIDAVVLSTQHNPDVKQSDLREAVMELIIKHSLPAELLHKDTQYHINPTGQFVIGGPVGD
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLADRCEIQV
SYAIGVAQPTSISLNTFGTGKLSDDKIIALVREHFDLRPYAITTMLDLLHPMYRATAAYG
HFGRTPYEMTVGADTFTAFTWERTDKADALRAAAGL