Protein Info for Psest_3263 in Pseudomonas stutzeri RCH2

Annotation: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 27 to 72 (46 residues), 29.8 bits, 9.4e-11
• TIGR01143: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
  • amino acids 30 to 450 (421 residues), 477 bits, E=2.7e-147
• PF08245: Mur_ligase_M
  • amino acids 107 to 299 (193 residues), 158.5 bits, E=3.2e-50
• PF02875: Mur_ligase_C
  • amino acids 319 to 384 (66 residues), 40.8 bits, E=3.3e-14

Best Hits

Swiss-Prot: 46% identical to MURF_ECOLI: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (murF) from Escherichia coli (strain K12)

KEGG orthology group: K01929, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase [EC: 6.3.2.10] (inferred from 90% identity to psa:PST_1078)

MetaCyc: 46% identical to D-alanyl-D-alanine-adding enzyme (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase. [EC: 6.3.2.10]

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.10)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.10

Sequence Analysis Tools

See L0GQT4 at UniProt or InterPro

Protein Sequence (458 amino acids)

>Psest_3263  UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (Pseudomonas stutzeri RCH2)
MLDAMRLSELQQPLSGRLVGADVSFAAVSTDSRAIEPGQLFIALTGPRFDGHAYLADVAA
KGAVAALVEREIETSTLPQLVVADTRLALGLLGASNRNAFNGPVAAITGSSGKTSVKEML
ASILRSAYGQNAVLATRGNLNNDLGAPLTLLELCDEHRAAVIELGASRPGDIAYTVKLVR
PDVSLITNAGSAHVGEFGGPHKIVEAKGEILDGLSESGIAVLNRDDKAYPIWAERVAGKR
IVSFGLDNPLADFSAGSIAYDQRGCPSFVLTGPLGNLRIQLNLLGRHNVANALAAAAAAY
ALGVDADAVVAGLQQLQPVKGRAVAQMLANGVRLIDDSYNANPASICAAIDVLASFAGRT
ILVLGDMGELGEWAEQGHREVGAYAVGKVDTLYAVGPLMAHAVQAFGPGARHFADQASLI
DSLRAEQGSGTTILIKGSRSAAMDKVVDALAGPSMEKH