Protein Info for Psest_0032 in Pseudomonas stutzeri RCH2

Annotation: D-alanyl-D-alanine carboxypeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 312 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00768: Peptidase_S11" amino acids 28 to 256 (229 residues), 250 bits, see alignment E=2.3e-78 PF13354: Beta-lactamase2" amino acids 39 to 178 (140 residues), 48.6 bits, see alignment E=6.9e-17

Best Hits

Swiss-Prot: 68% identical to PBP5_PSEAE: D-alanyl-D-alanine endopeptidase (pbpG) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K07262, D-alanyl-D-alanine endopeptidase (penicillin-binding protein 7) [EC: 3.4.21.-] (inferred from 95% identity to psa:PST_0030)

MetaCyc: 67% identical to peptidoglycan DD-endopeptidase PbpG (Escherichia coli K-12 substr. MG1655)
3.4.-.-

Predicted SEED Role

"Murein-DD-endopeptidase (EC 3.4.99.-)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.99.-)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-, 3.4.99.-

Use Curated BLAST to search for 3.4.21.- or 3.4.99.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GGU2 at UniProt or InterPro

Protein Sequence (312 amino acids)

>Psest_0032 D-alanyl-D-alanine carboxypeptidase (Pseudomonas stutzeri RCH2)
MNFRRTLASLFVVCLASLPVLHAQAAKPAVQELASGSALLVDLNTNEVLYSSNPDMVVPI
ASVTKLMTAIVALDAKLPLDQVLPVTIRDAKEMQGVFSRVRIGSEISRRELLLLTLMSSE
NRAAASLAHHYPGGYSAFIQAMNAKARALGMSRTYYVEPTGLSERNVSSANDLVKLIRAS
RQYPLIQQFSTTDEKTVAFRKPNYTLGFRNTNALVRKNNWDIQLSKTGFTNAAGHCLVMS
TTMKQRPVAFVVLDAFGKYTHMADANRLKKWLETGTVTPVPAAALAYKQQKQAQRQLAGQ
PADAAQALLDAR