Protein Info for Psest_3258 in Pseudomonas stutzeri RCH2

Annotation: UDP-N-acetylmuramate--alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01082: UDP-N-acetylmuramate--L-alanine ligase
  • amino acids 20 to 469 (450 residues), 557.7 bits, E=1.1e-171
• PF01225: Mur_ligase
  • amino acids 20 to 118 (99 residues), 103.1 bits, E=1.3e-33
• PF08245: Mur_ligase_M
  • amino acids 123 to 303 (181 residues), 88.9 bits, E=6.6e-29
• PF02875: Mur_ligase_C
  • amino acids 325 to 460 (136 residues), 55.1 bits, E=2.2e-18

Best Hits

Swiss-Prot: 98% identical to MURC_PSEU5: UDP-N-acetylmuramate--L-alanine ligase (murC) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K01924, UDP-N-acetylmuramate--alanine ligase [EC: 6.3.2.8] (inferred from 98% identity to psa:PST_1083)

MetaCyc: 61% identical to UDP-N-acetylmuramate--L-alanine ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramate--L-alanine ligase. [EC: 6.3.2.8]

Predicted SEED Role

"UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.8)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.8

Sequence Analysis Tools

See L0GQS6 at UniProt or InterPro

Protein Sequence (485 amino acids)

>Psest_3258 UDP-N-acetylmuramate--alanine ligase (Pseudomonas stutzeri RCH2)
MAKSPAAVKAEVRRMRRIRRIHFVGIGGVGMCGIAEVLLNLGYEVSGSDLKESASTDRLQ
SFGAQIFIGHQAGNVAGADVLVVSSAVNSANPEVALALEQRIPVVPRAEMLAELMRYRHG
IAVAGTHGKTTTTSLLASVFAAGGLDPTFVIGGRLTAAGTNAQLGSSRYLIAEADESDAS
FLHLQPMVAVVTNIDADHMSTYGGDFGKLKKTFVEFLHNLPFYGLAVLCVDDPVVREIIP
QIGRPTTTYGFSEDADVRAINVRQEGMRTYFTVLRDGCEPLDVSVHMPGNHNVLNALATI
AIATDEGIDDEAIIQGLAEFAGVGRRFQVYGDLPVENGSVMLVDDYGHHPREVAAVIKAV
RGGWPERRLVMVYQPHRYSRTRDLYDDFVQVLGESNVLLLMEVYPAGEDPIPGADSRQLC
HSIRQRGQLDPIYVERGVDLAPLVKPLLRAGDILLCQGAGDIGGLAPQLLRSPLFGGDGS
TRKPQ