Protein Info for Psest_3221 in Pseudomonas stutzeri RCH2
Annotation: Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00666, fatty-acyl-CoA synthase [EC: 6.2.1.-] (inferred from 96% identity to psa:PST_1119)Predicted SEED Role
"Medium-chain-fatty-acid--CoA ligase (EC 6.2.1.-)" (EC 6.2.1.-)
KEGG Metabolic Maps
- Alkaloid biosynthesis II
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Caprolactam degradation
- Ethylbenzene degradation
- Geraniol degradation
- Limonene and pinene degradation
- Propanoate metabolism
- Tyrosine metabolism
- alpha-Linolenic acid metabolism
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.-
Use Curated BLAST to search for 6.2.1.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GQP2 at UniProt or InterPro
Protein Sequence (561 amino acids)
>Psest_3221 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II (Pseudomonas stutzeri RCH2) MLQTRVIKPAANAYQYPLLIKRLLLSGVRYERTREIVYRDQLRYDYRTLNQRVARLANVL TAAGVKAGDTVAVMDWDSHRYLECMFAIPMIGAVVHTINVRLSPDQILYTMNHADDRFVL VNSEFVPLYQAIAGQLTTVQKTLLLTDGDVHDAGLPDCVGEYESLLAAAAPSYDFPDFDE DSVATTFYTTGTTGNPKGVYFTHRQLVLHTLAAAVTVGCRENPRLMGSDDVYMPITPMFH VHAWGLPYVATMLGLKQVYPGRYDPEYLIDLWRREQVTFSHCVPTIVQMLLNAKAAQGTD FKGWKITIGGSALTRGLYDQAKARGMNLIAAYGMSETCPLISGAHINDELLEADEDTRST FHLKAGVPVVLVDAAIQAADGSFLPADGTSQGELVLRAPWLTQGYYNEPEKSEELWADGW LHTGDVAVIDEMANIEIRDRIKDVIKTGGEWLSSLTLEGLISRHEAVRDVAVVGVPDERW GERPFALVVLAEGRELNADQLQTFLEPAVAEGHINKWAIPQQVAVVSEIPKTSVGKLDKK RIRSEIARWQEDAAVAQPSPS