Protein Info for Psest_0313 in Pseudomonas stutzeri RCH2

Annotation: 5,10-methylenetetrahydrofolate reductase, prokaryotic form

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 45% identical to METF_AQUAE: 5,10-methylenetetrahydrofolate reductase (metF) from Aquifex aeolicus (strain VF5)

KEGG orthology group: K00297, methylenetetrahydrofolate reductase (NADPH) [EC: 1.5.1.20] (inferred from 95% identity to psa:PST_3936)

MetaCyc: 40% identical to 5,10-methylenetetrahydrofolate reductase (Escherichia coli K-12 substr. MG1655)
RXN-22438 [EC: 1.5.1.54]

Predicted SEED Role

"5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20)" in subsystem Methionine Biosynthesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 1.5.1.20)

MetaCyc Pathways

folate transformations III (E. coli) (9/9 steps found)
folate transformations II (plants) (10/11 steps found)
folate transformations I (9/13 steps found)
reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (3/10 steps found)
Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.20 or 1.5.1.54

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHY6 at UniProt or InterPro

Protein Sequence (309 amino acids)

>Psest_0313 5,10-methylenetetrahydrofolate reductase, prokaryotic form (Pseudomonas stutzeri RCH2)
MLASRSDLQRLEDVLGAPRPTTLDSPMSTSPRPSISFEFFPTKTDAGHEKLLNVARQLAG
YQPDFFSCTYGAGGSTRDRTLNTVLQLDGEVKVPTAPHLSCVGDTKAELRELLNIYKDAG
IQRIVALRGDLPSGMGMASGELRYANELVEFIRSETGDHFHIEVAAYPEMHPQARNFEDD
LANFVRKAKAGANSAITQYFFNADCYFYFVERVRKLGVDTPIVPGIMPITNYSKLARFSD
ACGAEIPRWVRKQLEAYGDDMQSIQAFGEQVITEMCQKLLAGDAPGLHFYTLNQAEPSLA
IWNNLGLSR