Protein Info for PGA1_c31680 in Phaeobacter inhibens DSM 17395

Annotation: putative bifunctional enzyme FolC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 84% identity to sit:TM1040_2675)

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17)" (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

folate polyglutamylation (5/5 steps found)
superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
tetrahydrofolate biosynthesis I (3/3 steps found)
superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
tetrahydrofolate biosynthesis II (2/3 steps found)
superpathway of chorismate metabolism (36/59 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7F361 at UniProt or InterPro

Protein Sequence (422 amino acids)

>PGA1_c31680 putative bifunctional enzyme FolC (Phaeobacter inhibens DSM 17395)
MTQTSDAILARMMALHPKIIDLTLDRVWRLLAALDNPQDKLPPVIHLAGTNGKGSTQAMI
RAGLEGGGKSVHAYTSPHLARFHERIRLAGELISEQHLSEVLDECYAKNGGENITYFEIT
TVAGLLAVSRMPADYTLLEVGLGGRLDATNVITPEVSVITPISIDHEQFLGNTLAKIAAE
KAGIIKRGVPVVVGPQPDEAMEVIEATAARLGAPLIAYGQHWHVWEERGRLVFQDEAGLL
DLPLPALLGAHQIQNAGAALAVLRHMGADEAACEAAMTNAEWPARMQKLKTGPLVDVAPE
AELWLDGGHNAAAGLALAKVLAGLPARPTHLICGMLNTKDVSGYMAPLAPHVESLTAISI
PDEVNTLSAEETEAAAKSVGIAAGTAESTIAGLQAIIAKDPHARVLICGSLYLAGHILRD
NG