Protein Info for Psest_3162 in Pseudomonas stutzeri RCH2

Annotation: amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 394 transmembrane" amino acids 20 to 41 (22 residues), see Phobius details amino acids 85 to 112 (28 residues), see Phobius details amino acids 131 to 152 (22 residues), see Phobius details amino acids 183 to 203 (21 residues), see Phobius details amino acids 216 to 240 (25 residues), see Phobius details amino acids 255 to 277 (23 residues), see Phobius details amino acids 336 to 354 (19 residues), see Phobius details amino acids 364 to 385 (22 residues), see Phobius details TIGR01726: amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family" amino acids 85 to 148 (64 residues), 59.5 bits, see alignment E=1.9e-20 PF00528: BPD_transp_1" amino acids 205 to 389 (185 residues), 61.9 bits, see alignment E=3.4e-21

Best Hits

Swiss-Prot: 61% identical to YHDX_ECOLI: Putative amino-acid ABC transporter permease protein YhdX (yhdX) from Escherichia coli (strain K12)

KEGG orthology group: K09970, general L-amino acid transport system permease protein (inferred from 71% identity to pmy:Pmen_3559)

Predicted SEED Role

"Glutamate Aspartate transport system permease protein GltJ (TC 3.A.1.3.4)" (TC 3.A.1.3.4)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPE2 at UniProt or InterPro

Protein Sequence (394 amino acids)

>Psest_3162 amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (Pseudomonas stutzeri RCH2)
MRSIARGAVPRRSPFTDPRIRAWVFQVLAVLAVVAFGWYLFHNTQTNLAHRGITSGFGFL
NNAAGFGISQHLIDYSESDTYGRVFWVGLLNTLLVSVIGIFLATVMGFILGVGRLSSNWL
IRQLATLYIEIFRNIPPLLLIFFVYFAVISPLPGPRNSLSLWDVVFVNNRGVQMPAPSAG
DGFWAFWLALLVALVAVVVLNRWARARRHATGRMFPVFWTSPGLFLAIPWLMTFIAGAPF
TWEVPELQRFNIRGGWVVIPELVSIVVALSVYTAAFIGETVRSGIQSVSHGQTEAAASLG
LRPGQVLRLVIVPQALRVIVPPLTSQYLNLAKNSSLAAAIGYPDMVSLFAGTVLNQTGQA
IETMAITMSVYLAISISISLLMNWYNKRIALIER