Protein Info for Psest_3159 in Pseudomonas stutzeri RCH2

Annotation: phosphoribosylformylglycinamidine synthase, single chain form

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1298 TIGR01735: phosphoribosylformylglycinamidine synthase" amino acids 3 to 1297 (1295 residues), 2151.4 bits, see alignment E=0 PF18076: FGAR-AT_N" amino acids 35 to 148 (114 residues), 139.4 bits, see alignment E=1.3e-44 PF18072: FGAR-AT_linker" amino acids 169 to 218 (50 residues), 82.6 bits, see alignment (E = 5.6e-27) PF02769: AIRS_C" amino acids 430 to 587 (158 residues), 114.3 bits, see alignment E=1.6e-36 amino acids 839 to 970 (132 residues), 55.4 bits, see alignment E=2.1e-18 PF22689: FGAR-AT_PurM_N-like" amino acids 646 to 805 (160 residues), 284.8 bits, see alignment E=4.1e-89 PF13507: GATase_5" amino acids 1042 to 1297 (256 residues), 371.4 bits, see alignment E=4.9e-115

Best Hits

Swiss-Prot: 67% identical to PUR4_IDILO: Phosphoribosylformylglycinamidine synthase (purL) from Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR)

KEGG orthology group: K01952, phosphoribosylformylglycinamidine synthase [EC: 6.3.5.3] (inferred from 64% identity to abo:ABO_0785)

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GQR8 at UniProt or InterPro

Protein Sequence (1298 amino acids)

>Psest_3159 phosphoribosylformylglycinamidine synthase, single chain form (Pseudomonas stutzeri RCH2)
MLILRGAPALSAFRHGKLLAQLTDKVPAVSGLYAEFAHFAEVSGTLGADEQNVLTRLLKY
GPSVPVQEPAGRLFLVVPRFGTISPWSSKASDIAHNCGLEKIQRLERGIAYYVQGEFSDG
DAQLIAAALHDRMTQLVLNRFEEAANLFSHAEPKPLTAVDILGGGRAALEKANTELGLAL
AEDEIDYLVSAFQGLKRNPHDIELMMFAQANSEHCRHKIFNASWDIDGESQDKSLFGMIK
NTYQMHSENVLSAYKDNASVIVGHTAGRFFPNPETRQYGAVQEPVHILMKVETHNHPTAI
SPFSGASTGSGGEIRDEGATGRGAKPKAGLTGFTVSNLNIPGFEQPWEQAYGKPERIVTP
LDIMIEGPLGGAAFNNEFGRPALTGYFRTFEQSISTPRGDEVRGYHKPIMLAGGLGNIRE
DHVQKAEITVGAKLIVLGGPAMLIGLGGGAASSVATGASSADLDFASVQRENPEMERRCQ
EVIDRCWQLGDQNPIAFIHDVGAGGISNAFPELVNDGGRGGRFELRNVPNDEPGMAPHEI
WSNESQERYVLAVSTVDFERFQAICERERCPFAVVGEATEEPQLTVTDSHFGNTPVDMPL
EVLLGKPPRMHRSASREAELGDDFDAAAVDLSEAVTRVLRHPAVASKSFLITIGDRSITG
QVARDQMVGPWQVPVADCAVTATSYDVYTGEAMAMGERTPLALLDAPASGRMAIGETLTN
LAAARIEKISDIKLSANWMAAAGHPGEDARLYETVRAVGMELCPQLGLTIPVGKDSMSMK
TRWSEEGAEKSVTSPMSLIVSGFAPVTDVRQTLTPQLRLDKGATDLILIDLGRGQNRMGA
SILAQVYGQLGRQAPDVDDAEDLQAFFAVVQGLNADGLLLAYHDRSDGGLLTTVLEMAFA
GHCGLSLNLDGLLESAVDVAPMLFNEELGAVIQVRQGDTEIVLAQFSAAGLGDCVAVIGQ
PVNNGHVSIKHGENEVFAGERRLLQRQWAETSYQIQRLRDNAECADQEFDALLEEDNPGL
SAKLSFDVNEDISAPYIKRGVRPQIAVLREQGVNGQVEMAAAFDRAGFAAVDVHMSDILS
GRVSLEEFKGLVACGGFSYGDVLGAGEGWAKSVLFNARARDGFQAFFERKDSFALGVCNG
CQMMSNLHELIPGTENWPHFVRNRSEQFEARVAMVQVQDSPSIFLQGMAGSRLPIAIAHG
EGHAEFESEEAMLQADLSGTVALRFVDNHGKVTERYPANPNGSPRGITGLSSRDGRVTIM
MPHPERVFRAVTNSWRPDEWQEDGGWMRMFRNARVWVD