Protein Info for HP15_3040 in Marinobacter adhaerens HP15

Updated annotation (from data): Arginine N-succinyltransferase (EC 2.3.1.109)
Rationale: Specifically important for utilizing L-Arginine. Automated validation from mutant phenotype: the predicted function (2.3.1.109) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: arginine N-succinyltransferase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04958: AstA
  • amino acids 1 to 340 (340 residues), 431.7 bits, E=7e-134
• TIGR03243: arginine and ornithine succinyltransferase subunits
  • amino acids 3 to 342 (340 residues), 426.7 bits, E=5.4e-132
• TIGR03244: arginine N-succinyltransferase
  • amino acids 3 to 341 (339 residues), 442.6 bits, E=7.3e-137

Best Hits

KEGG orthology group: K00673, arginine N-succinyltransferase [EC: 2.3.1.109] (inferred from 79% identity to maq:Maqu_3317)

Predicted SEED Role

"Arginine N-succinyltransferase (EC 2.3.1.109)" in subsystem Arginine and Ornithine Degradation (EC 2.3.1.109)

MetaCyc Pathways

• L-arginine degradation II (AST pathway) (5/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.109

Use Curated BLAST to search for 2.3.1.109

Sequence Analysis Tools

See E4PNX4 at UniProt or InterPro

Protein Sequence (366 amino acids)

>HP15_3040 Arginine N-succinyltransferase (EC 2.3.1.109) (Marinobacter adhaerens HP15)
MLVIRPLQENDLDDLYAMAQSAGKGLTTLPADRELLQKKINHARETFNQRIAPEAGLYLF
ALEDTERKKTVGISGIQARVGLDEVFYNYRLSVTVNASKELGVHVRTPTLHLSNDMTDTS
EICSLLLSDEYKGGGSGLLLSRCRFMYLDEFRKHFSEKIFAEMRGVSDANGRSPLWDALG
TKFFDMEFSEADMLSGLGNKSFIAELMPKYPIYLSMLPDSARAVIGRVHDNTAPALRMLQ
SEGFNFNGLVDIFDGGPVVEAFVHNVRTVREGMNRHAMVTRKPVNLDVPSEERVMVSNRS
FRDFRVTTVPIDCIGPDTVSLPPEVAEALQIESGDPVRLAPLKDSGLLTKHSYRSSVPGG
ASKWQS