Protein Info for Psest_0310 in Pseudomonas stutzeri RCH2

Annotation: The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1045 signal peptide" amino acids 10 to 11 (2 residues), see Phobius details transmembrane" amino acids 12 to 28 (17 residues), see Phobius details amino acids 339 to 359 (21 residues), see Phobius details amino acids 366 to 389 (24 residues), see Phobius details amino acids 395 to 413 (19 residues), see Phobius details amino acids 440 to 460 (21 residues), see Phobius details amino acids 471 to 496 (26 residues), see Phobius details amino acids 540 to 558 (19 residues), see Phobius details amino acids 872 to 890 (19 residues), see Phobius details amino acids 897 to 917 (21 residues), see Phobius details amino acids 923 to 947 (25 residues), see Phobius details amino acids 972 to 993 (22 residues), see Phobius details amino acids 1001 to 1026 (26 residues), see Phobius details PF00873: ACR_tran" amino acids 1 to 1026 (1026 residues), 1348.2 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1043 (1043 residues), 1788.4 bits, see alignment E=0 PF03176: MMPL" amino acids 297 to 514 (218 residues), 35.3 bits, see alignment E=1e-12

Best Hits

Swiss-Prot: 78% identical to MEXB_PSEAE: Multidrug resistance protein MexB (mexB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 64% identity to aha:AHA_2910)

MetaCyc: 63% identical to multidrug efflux pump RND permease AcrF (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-352; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-367

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GFY7 at UniProt or InterPro

Protein Sequence (1045 amino acids)

>Psest_0310 The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family (Pseudomonas stutzeri RCH2)
MSRFFIDRPIFAWVIALVIMLAGGLSILKLPVNQYPSIAAPAVGISVSYPGASAQTVQDT
VVQVIEQQLNGIDGLRYISSESNSDGSMTITVTFDQGTNPDIAQVQVQNKLQLATPLLPQ
EVQQQGIRVTKAVRNFLIVIGLVSTDGSLDQRDLANYIVANMQDPISRTKGVGDFQVFGA
QYAMRIWLDPAKLNSFSMTPVDVSTAIQAQNVQISSGQLGGLPALPGQQLNATVIGKTRL
QTPEQFRNILLKVNSDGSQVRLGDVARVELGGETYAVSAHFNGKPAAGLALKLATGANAL
DTVEGVRKTIEELKPFFPPGVEVVYPYDTTPVISASITGVMHTLLEAFVLVFLVMYLFLQ
NFRATLVPTLAVPVVLLGTFGVLAVFGFSINTLTMFAMILAIGLLVDDAIVVVENVERVM
REEGLSPLEATRKSMGQIQGALIGIGVVLSAVLLPMAFFGGSTGVIYRQFSITIVSAMVL
SVLMALIFTPALCATLLKPIDKDDHHEKRGFFGWFNRTFERSVNGYERGVKAVLKRKAPF
LLLYVLIVGVMVVLFTRIPSAFLPEEDQGVLFAQVMTPSGSTAERTQQSIDAMRSYLLEE
EGDIVRSVFTVTGFSFAGRGQSSGIAFIGLKPWSERTNKEDSVFALAQRAQQHFASFRDA
QVFAFAPPAVMELGNATGFNFFLQDRAGVGQQVLQQARDKFMQLASQSPVLTRVRPNTLR
DEPQYQVLIDDEKARVLGISLAEINSTLSIAWGGRYVNDFIDRGRVKKVYLQGVADARMN
PEDLAKWYVRNDAGEMVPFSAFASGEWTYGSPKLSRYNGVSAIEMLGEPAPGYSSGDAMA
EVERIVAQLPPGVGYSWTGQSYEERLAGSQTLALYALSLLVVFLCLAALYESWSIPFSVM
LVVPLGIVGALAFTMLRGLSNEVFFQVGLVTTIGLSARNAILIVEFAKALHEQGMSYADA
ALQACRMRLRPIIMTSLAFILGVVPLAVASGAGAGSKHAIGTGVIGGMLTAAVLAIFWIP
LFYVMICTFFDRTKPANQAAKEESL