Protein Info for PGA1_c31400 in Phaeobacter inhibens DSM 17395
Annotation: 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase HpcH
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 52% identical to HPCH_ECOHS: 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH) from Escherichia coli O9:H4 (strain HS)
KEGG orthology group: K02510, 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase [EC: 4.1.2.-] (inferred from 81% identity to sil:SPO3686)MetaCyc: 52% identical to 4-hydroxy-2-ketopimelate aldolase monomer (Escherichia coli C)
4-HYDROXY-2-KETOPIMELATE-LYSIS-RXN [EC: 4.1.2.52]
Predicted SEED Role
"2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4)" (EC 4.1.2.n4)
MetaCyc Pathways
- 4-hydroxyphenylacetate degradation (5/8 steps found)
KEGG Metabolic Maps
- Fructose and mannose metabolism
- Lysine degradation
- Naphthalene and anthracene degradation
- Pentose phosphate pathway
- Phenylalanine, tyrosine and tryptophan biosynthesis
- Tyrosine metabolism
- alpha-Linolenic acid metabolism
Isozymes
Compare fitness of predicted isozymes for: 4.1.2.-, 4.1.2.n4
Use Curated BLAST to search for 4.1.2.- or 4.1.2.52 or 4.1.2.n4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See I7F0R1 at UniProt or InterPro
Protein Sequence (256 amino acids)
>PGA1_c31400 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase HpcH (Phaeobacter inhibens DSM 17395) MPAPKNIFKQALANGDRQIGCWMSFGEAAVAEVMGTCGFDWLVIDGEHAPNDIRSIRDQL MALAASDSHPVVRVPVGETWIIKQVLDAGAQTVLVPIVEDADQARELVRACQYPPHGTRG VGATAARATLFGTASDYIQTADQQICLLLQVENRKGIENLNEILAVPGVDGIFIGPADLS TDMGFEGNSAHPEVRAVIADALARIKAAGKAPGILGTSPEATQAYFDMGAQFLAVGIDVM LLAKSARALATDWTSK