Protein Info for Psest_3113 in Pseudomonas stutzeri RCH2

Annotation: electron transport complex, RnfABCDGE type, B subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 191 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR01944: electron transport complex, RnfABCDGE type, B subunit" amino acids 1 to 185 (185 residues), 281.8 bits, see alignment E=1.1e-88 PF04060: FeS" amino acids 44 to 75 (32 residues), 51 bits, see alignment 2.4e-17 PF14697: Fer4_21" amino acids 107 to 161 (55 residues), 68.2 bits, see alignment E=1.3e-22 PF00037: Fer4" amino acids 108 to 129 (22 residues), 26.8 bits, see alignment (E = 8.3e-10) PF13237: Fer4_10" amino acids 108 to 155 (48 residues), 33.7 bits, see alignment E=7.2e-12

Best Hits

Swiss-Prot: 100% identical to RNFB_PSEU5: Ion-translocating oxidoreductase complex subunit B (rnfB) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K03616, electron transport complex protein RnfB (inferred from 100% identity to psa:PST_1211)

Predicted SEED Role

"Electron transport complex protein RnfB" in subsystem Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GQM4 at UniProt or InterPro

Protein Sequence (191 amino acids)

>Psest_3113 electron transport complex, RnfABCDGE type, B subunit (Pseudomonas stutzeri RCH2)
MSAVLIAVLALLALCLLGGAILGFAAVRFRVEGDPIAEQINALLPQTQCGQCGYPGCKPY
AEAIAGGDKINKCPPGGEATIQALADLLDVEAEPLDAEGGEKPQMVAYIREAECIGCTKC
IQACPVDAIVGAARQMHTVIISECTGCDLCVEPCPVDCIDMIEVGSNLQSWKWNRPLAPG
QLIATDREQAA