Protein Info for Psest_3099 in Pseudomonas stutzeri RCH2

Annotation: Negative regulator of sigma E activity

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 192 transmembrane" amino acids 84 to 104 (21 residues), see Phobius details PF03872: RseA_N" amino acids 7 to 77 (71 residues), 49.7 bits, see alignment E=4.7e-17 PF03873: RseA_C" amino acids 120 to 172 (53 residues), 36.8 bits, see alignment E=3.9e-13

Best Hits

Swiss-Prot: 68% identical to MUCA_PSEAE: Sigma factor AlgU negative regulatory protein (mucA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03597, sigma-E factor negative regulatory protein RseA (inferred from 92% identity to psa:PST_1224)

Predicted SEED Role

"Sigma factor RpoE negative regulatory protein RseA" in subsystem Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GQD2 at UniProt or InterPro

Protein Sequence (192 amino acids)

>Psest_3099 Negative regulator of sigma E activity (Pseudomonas stutzeri RCH2)
MSREALHESLSAVMDNEADELELRRVLAGDNPELRATWSRYQLARAAMHKELIEPRLDIA
SAVSAALADEAAPVKTQRNGWKSLGRLAVAASVTVAVLAGVRLYNQNEVAGPQLAQQAPQ
PSIAVPQVNQGPTVLAGYSEQAEAPAQAGAPVERWHEERLPSYVRQHAQQAAFSNGSESA
LPYARAASMEER