Protein Info for Psest_3096 in Pseudomonas stutzeri RCH2

Annotation: periplasmic serine protease, Do/DeqQ family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 471 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR02037: peptidase Do" amino acids 28 to 467 (440 residues), 526.3 bits, see alignment E=3.1e-162 PF00089: Trypsin" amino acids 93 to 252 (160 residues), 63.1 bits, see alignment E=1.1e-20 PF13365: Trypsin_2" amino acids 96 to 229 (134 residues), 129.7 bits, see alignment E=4.4e-41 PF00595: PDZ" amino acids 265 to 326 (62 residues), 39.8 bits, see alignment E=1.4e-13 amino acids 379 to 454 (76 residues), 32 bits, see alignment E=3.9e-11 PF13180: PDZ_2" amino acids 270 to 358 (89 residues), 58.1 bits, see alignment E=2.7e-19 amino acids 382 to 465 (84 residues), 36 bits, see alignment E=2.1e-12 PF17820: PDZ_6" amino acids 295 to 348 (54 residues), 47.1 bits, see alignment 4.8e-16

Best Hits

Swiss-Prot: 81% identical to DEGPL_PSEMY: Probable periplasmic serine endoprotease DegP-like (Pmen_1471) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: None (inferred from 96% identity to psa:PST_1227)

Predicted SEED Role

"HtrA protease/chaperone protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GP80 at UniProt or InterPro

Protein Sequence (471 amino acids)

>Psest_3096 periplasmic serine protease, Do/DeqQ family (Pseudomonas stutzeri RCH2)
MFNRNGCFAFVAGLALLGQATLAQADLPDFTPLVESASPAVVNISTKQKVPARGATAQMP
ELEGLPPIFREFFEHSIPQMPGAPGRGQQREAQSLGSGFIISDDGYVLTNNHVVAGADEI
IVRLPDRSELEAKLIGADPRSDVAVLKVEGKGLPTVKIGRSDELKAGEWVLAIGSPFGFD
HTVTAGIVSATGRSLPNESYVPFIQTDVAINPGNSGGPLFNLKGEVVGINSQIFTRSGGF
MGLSFAIPIDVAMDVANQLRTDGKVNRGWLGVVIQEVNKDLAESFGLERPAGALVAQVMD
GGPAARSGLRVGDVILSLNGKPIVMSADLPHLVGALKPGSKARMEVVRDGDRKMLDVSIG
AMPEEGEPVAASGGGQERSDNRLGVKVTELTEEQKKSLDLPGGVVITEILNGPAAMIGLR
PGDVITHLNNQAINSAATFGRVAEQLPKNRSVSMRVLRQGRASFITFKLAE