Protein Info for Psest_3091 in Pseudomonas stutzeri RCH2

Annotation: GTP-binding protein Era

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00231: small GTP-binding protein domain
  • amino acids 14 to 175 (162 residues), 105.5 bits, E=2.6e-34
• TIGR00436: GTP-binding protein Era
  • amino acids 15 to 285 (271 residues), 328 bits, E=4.5e-102
• PF01926: MMR_HSR1
  • amino acids 17 to 131 (115 residues), 90.3 bits, E=4.3e-29
• PF02421: FeoB_N
  • amino acids 17 to 173 (157 residues), 53.1 bits, E=1.2e-17
• PF04548: AIG1
  • amino acids 18 to 125 (108 residues), 38.6 bits, E=3.4e-13
• PF00009: GTP_EFTU
  • amino acids 82 to 180 (99 residues), 27 bits, E=1.5e-09
• PF07650: KH_2
  • amino acids 215 to 290 (76 residues), 74.4 bits, E=2.3e-24

Best Hits

Swiss-Prot: 87% identical to ERA_PSEA8: GTPase Era (era) from Pseudomonas aeruginosa (strain LESB58)

KEGG orthology group: K03595, GTP-binding protein Era (inferred from 97% identity to psa:PST_1232)

MetaCyc: 60% identical to 30S ribosomal subunit maturation GTPase Era (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein Era" in subsystem Bacterial Cell Division or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See L0GP76 at UniProt or InterPro

Protein Sequence (303 amino acids)

>Psest_3091 GTP-binding protein Era (Pseudomonas stutzeri RCH2)
MTDEQLQPEDVSRCGYVAIVGRPNVGKSTLLNHILGQKLAITSRKPQTTRHNMLGIKTEG
SVQAVYVDTPGLHKNGETALNRYMNRTAASALKDVDVVIFVVDRTRWTDEDQAVLERVQY
VTGPLIIAVNKTDRVEDKAELLPHLRWLAEQLPNAEIVPISAQHGQNLETLEKLVADRLP
ESEHFFPEDQITDRSSRFLAAELVREKIMRQLGAEVPYQITVEIEDFKQEGRVLHIHALI
LVERDGQKKIIIGDKGERIKRIGQEARKDMEVLFDSKVMLHLWVKVKGGWSDDERALHSL
GYQ