Protein Info for Psest_3081 in Pseudomonas stutzeri RCH2

Annotation: ATP-dependent protease La

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 795 PF02190: LON_substr_bdg" amino acids 31 to 226 (196 residues), 88.1 bits, see alignment E=2.4e-28 TIGR00763: endopeptidase La" amino acids 33 to 795 (763 residues), 918.8 bits, see alignment E=1.8e-280 PF07728: AAA_5" amino acids 377 to 510 (134 residues), 32.5 bits, see alignment E=2.4e-11 PF00004: AAA" amino acids 377 to 516 (140 residues), 88.6 bits, see alignment E=1.5e-28 PF22667: Lon_lid" amino acids 539 to 589 (51 residues), 63.3 bits, see alignment 4.1e-21 PF05362: Lon_C" amino acids 594 to 794 (201 residues), 264.8 bits, see alignment E=1.5e-82 PF13541: ChlI" amino acids 643 to 764 (122 residues), 32.3 bits, see alignment E=2.4e-11

Best Hits

Swiss-Prot: 64% identical to LON2_HYDCU: Lon protease 2 (lon2) from Hydrogenovibrio crunogenus (strain XCL-2)

KEGG orthology group: None (inferred from 96% identity to psa:PST_1240)

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.53

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GP67 at UniProt or InterPro

Protein Sequence (795 amino acids)

>Psest_3081 ATP-dependent protease La (Pseudomonas stutzeri RCH2)
MNDDVNQDHIEQEIISSNGLVLPDQQLPDKLYIIPVHNRPFFPAQVLPVIVNEDPWAETL
ERVAKTPHQRVALFFVDSPVLDMATFDPDSLPEHGTMVRVHHATQEGGKLQFVAQGLARV
RIRGWLRRKPPYLVEVDYPKSDDDPRDEVKAYGMALINAIKELLPLNPLYSEELKNYLNR
FSPNDPSPLSDFAAALTTAPGVELQEVLDTVPVLKRMEKVLPLLRKEVEVARLQKELTGE
VNRKIGERQREFFLKEQLKIIQRELGITKDDKSADADEFRARLEGKVVPPAAQKRIDEEL
NKLSILETGSPEYAVTRNYLDWATALPWGVYGQDKLDLKRARKVLDKHHAGLDDIKNRIL
EFLAVGAFKGEIAGSIVLLVGPPGVGKTSIGKSIAESLGRPFYRFSVGGMRDEAEIKGHR
RTYIGALPGKLVQALKDVEVMNPVIMLDEIDKLSSSYQGDPASALLETLDPEQNVEFLDH
YLDLRLDLSKVLFVCTANTLDSIPGPLLDRMEVIRLSGYIAEEKLAIAKRHLWPKLLDKT
GVPKQRLAISDSAMKAVIEGYAREAGVRQLEKQLGKLVRKAVVQLLEDPQAVLKITPKDL
ESYLGKPVFRSEQVLSGVGVITGLAWTSMGGATLPIEATRIHTLNRGFKLTGKLGEVMKE
SAEIAYSYVSSNLKTFKGDPEFFDQAFVHLHVPEGATPKDGPSAGITMASALLSLARNQP
PKKGVAMTGELTLTGHVLPIGGVREKVIAARRQKIFELILPEANRGDFEELPDYLKEGLT
VHFAKRFADVAKVLF