Protein Info for Psest_3071 in Pseudomonas stutzeri RCH2

Annotation: Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01144: CoA_trans
  • amino acids 13 to 222 (210 residues), 174.8 bits, E=8.7e-56

Best Hits

Swiss-Prot: 89% identical to CATJ_PSEKB: 3-oxoadipate CoA-transferase subunit B (catJ) from Pseudomonas knackmussii (strain DSM 6978 / LMG 23759 / B13)

KEGG orthology group: K01040, glutaconate CoA-transferase, subunit B [EC: 2.8.3.12] (inferred from 92% identity to pfv:Psefu_2036)

Predicted SEED Role

"3-oxoadipate CoA-transferase subunit B (EC 2.8.3.6); Glutaconate CoA-transferase subunit B (EC 2.8.3.12)" (EC 2.8.3.12, EC 2.8.3.6)

MetaCyc Pathways

• catechol degradation III (ortho-cleavage pathway) (6/6 steps found)
• superpathway of salicylate degradation (6/7 steps found)
• 3-oxoadipate degradation (2/2 steps found)
• aromatic compounds degradation via β-ketoadipate (7/9 steps found)
• 4-methylcatechol degradation (ortho cleavage) (5/7 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (7/10 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (4/7 steps found)
• toluene degradation III (aerobic) (via p-cresol) (4/11 steps found)
• mandelate degradation to acetyl-CoA (9/18 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (11/35 steps found)
• superpathway of aerobic toluene degradation (7/30 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation
• Butanoate metabolism
• Styrene degradation

Isozymes

Compare fitness of predicted isozymes for: 2.8.3.12, 2.8.3.6

Use Curated BLAST to search for 2.8.3.12 or 2.8.3.6

Sequence Analysis Tools

See L0GP58 at UniProt or InterPro

Protein Sequence (265 amino acids)

>Psest_3071 Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit (Pseudomonas stutzeri RCH2)
MSAHLSNDYSTNEMMTVAAARNLGNGSVCFVGIGLPSKAANLARLTHAPEVVLIYESGPI
GAKPSVLPLSIGDGELAETADTVVPTGEIFRYWLQGGRIDVGFLGAAQVDKYGNINTTVI
GDYHKPKVRLPGAGGAPEIAGSAKKVLIILKQSHRTFVEKLAFITSVGHGEGGEHRKQLG
LPGAGPVAIITDLCIMEPEEGTNEFVVTSLHPGVAREQVIENTGWQIRFADNVAVTEAPR
AEELVALRALEARTAAAHGQLGGEE