Protein Info for Psest_0003 in Pseudomonas stutzeri RCH2

Annotation: DNA polymerase III, beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 PF00712: DNA_pol3_beta" amino acids 1 to 119 (119 residues), 143.8 bits, see alignment E=4.2e-46 TIGR00663: DNA polymerase III, beta subunit" amino acids 1 to 367 (367 residues), 367.6 bits, see alignment E=3.2e-114 PF02767: DNA_pol3_beta_2" amino acids 130 to 244 (115 residues), 139.6 bits, see alignment E=8.2e-45 PF02768: DNA_pol3_beta_3" amino acids 247 to 366 (120 residues), 141 bits, see alignment E=2.5e-45

Best Hits

Swiss-Prot: 87% identical to DPO3B_PSEAE: Beta sliding clamp (dnaN) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02338, DNA polymerase III subunit beta [EC: 2.7.7.7] (inferred from 99% identity to psa:PST_0002)

MetaCyc: 55% identical to beta sliding clamp (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"DNA polymerase III beta subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GF30 at UniProt or InterPro

Protein Sequence (367 amino acids)

>Psest_0003 DNA polymerase III, beta subunit (Pseudomonas stutzeri RCH2)
MHFSIQREALLKPLQLVAGVVERRQTLPVLSNVLLVVQGQQLSLTGTDLEVELVGRVALE
DAAEPGEITVPARKLMDICKSLPSDALISIRLDDQKIVIKSGRSRFTLSTLPANDFPTVE
EGPGSLSFSVEQGKLRKLIERSSFAMAQQDVRYYLNGMLIEVSNGMLRAVATDGHRLAMC
SMQAGIEQPDRHQVIVPRKGILELARLLNDQDAEVSIVLGQHHIRATTGEFTFTSKLVDG
KFPDYERVLPRGGDKLVVGDRQLLREAFSRTAILSNEKYRGIRLQLESGLLKIQANNPEQ
EEAEEEVAVDYNGGSLEIGFNVSYLLDVLGVMTTEQVRLILSDANSSALVQEFDNDDSAY
VVMPMRL