Protein Info for Psest_2996 in Pseudomonas stutzeri RCH2

Annotation: Rhodanese-related sulfurtransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 281 PF00581: Rhodanese" amino acids 8 to 131 (124 residues), 58.1 bits, see alignment E=5.2e-20 amino acids 160 to 273 (114 residues), 39.8 bits, see alignment E=2.7e-14

Best Hits

Swiss-Prot: 68% identical to THTM_PSEAE: Probable 3-mercaptopyruvate sulfurtransferase (sseA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01011, thiosulfate/3-mercaptopyruvate sulfurtransferase [EC: 2.8.1.1 2.8.1.2] (inferred from 75% identity to psa:PST_1362)

Predicted SEED Role

"Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1)" (EC 2.8.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.1, 2.8.1.2

Use Curated BLAST to search for 2.8.1.1 or 2.8.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GQ59 at UniProt or InterPro

Protein Sequence (281 amino acids)

>Psest_2996 Rhodanese-related sulfurtransferase (Pseudomonas stutzeri RCH2)
MPLAQLITTNQLAKRLHEPDLLILDCRFALEDPNYGECSYQQGHIPGAHFADLEKDLSDA
VVPGVTGRHPLPDPQRLLTRLQQCGLDEHSDVVLYDDGPGAFAARAWWLLVWLGKREGIY
LLDGGLKAWREAGLPLNDAIPANESGSFSGMPDSSMTVDASWLQDHLQSAALTMLDARAL
PRFLGEVEPIDPVAGHIPGAICAPFTENLGNTGCFLSPEQLQQRFAQLFGDKSPEAAVAY
CGSGVTACHNLFALCLAGYPLAKLYPGSWSEWITDPVRPRA