Protein Info for Psest_0295 in Pseudomonas stutzeri RCH2

Annotation: TonB family C-terminal domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 transmembrane" amino acids 21 to 43 (23 residues), see Phobius details PF13103: TonB_2" amino acids 199 to 272 (74 residues), 29 bits, see alignment E=9.4e-11 PF03544: TonB_C" amino acids 210 to 288 (79 residues), 62.7 bits, see alignment E=3.6e-21 TIGR01352: TonB family C-terminal domain" amino acids 212 to 289 (78 residues), 55.9 bits, see alignment E=2.2e-19

Best Hits

KEGG orthology group: K03832, periplasmic protein TonB (inferred from 96% identity to psa:PST_3955)

Predicted SEED Role

"Ferric siderophore transport system, periplasmic binding protein TonB" in subsystem Campylobacter Iron Metabolism or Hemin transport system or Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GFX2 at UniProt or InterPro

Protein Sequence (297 amino acids)

>Psest_0295 TonB family C-terminal domain (Pseudomonas stutzeri RCH2)
MNAAIRQPAPETTRVRPADRLGFTLFLAAALHLALILGLGFTLSEPKQISKTLEITLSTF
KSEEKPKEADFLAQDNQQGSGTLEHKASPKTTEQAPFQDTEVRKVTPPAAPPRSTQPEPA
KTVVTTRSRQPDKADTKQQTQPQPEPQAAPAFDSSQLSAEIASLEAELAHDVERYAKRPR
VSRQNSAATMRDISAWYRDEWRKKVERIGNLNYPDEARRQQIYGSLRMLVTINRDGTVQE
LRVIESSGQPVLDDAALRIVRLAAPFAPFTGELAQKFDQVEIIRTWRFERGDKLSSR